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TFF1 antibody [SPM313]

Anti-TFF1 antibody [SPM313] used in IHC (Paraffin sections) (IHC-P). GTX35100

Cat. No. GTX35100

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 SPM313

Isotype

 IgG1

Application

 IHC-P

Reactivity

 Human, Monkey
Package
100 μg ($529)
See all TFF1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
IHC-P 1-2μg/ml for 30 minutes at RT

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
Not tested in other applications.

Calculated MW

9 kDa. ( Note )

Product Note

It recognizes a polypeptide of 6.5kDa, identified as pS2 estrogen-regulated protein. Its epitope is localized between aa57-84 of human pS2 protein.

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide (around aa 57-84) of human pS2/TFF1 polypeptide (exact sequence is proprietary)

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

BCEI , D21S21 , HP1.A , HPS2 , TFF1 , pNR2 , pS2 , trefoil factor 1 , Trefoil factor 1 , Estrogen Inducible Protein pS2

Cellular Localization

Secreted

Background

Members of the trefoil family are characterized by having at least one copy of the trefoil motif, a 40-amino acid domain that contains three conserved disulfides. They are stable secretory proteins expressed in gastrointestinal mucosa. Their functions are not defined, but they may protect the mucosa from insults, stabilize the mucus layer, and affect healing of the epithelium. This gene, which is expressed in the gastric mucosa, has also been studied because of its expression in human tumors. This gene and two other related trefoil family member genes are found in a cluster on chromosome 21. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-TFF1 antibody [SPM313] used in IHC (Paraffin sections) (IHC-P). GTX35100

GTX35100 IHC-P Image

IHC-P analysis of human ovarian carcinoma tissue using GTX35100 TFF1 antibody [SPM313].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 529