Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1.38 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Full length human TFF1 Recombinant protein.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_11171325
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Members of the trefoil family are characterized by having at least one copy of the trefoil motif, a 40-amino acid domain that contains three conserved disulfides. They are stable secretory proteins expressed in gastrointestinal mucosa. Their functions are not defined, but they may protect the mucosa from insults, stabilize the mucus layer, and affect healing of the epithelium. This gene, which is expressed in the gastric mucosa, has also been studied because of its expression in human tumors. This gene and two other related trefoil family member genes are found in a cluster on chromosome 21. [provided by RefSeq]
Trefoil factor 1 antibody detects Trefoil factor 1 protein by Western blot analysis. Whole cell extracts (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Trefoil factor 1 antibody (GTX121461) diluted by 1:500.
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GTX121461 IHC-P Image
Trefoil factor 1 antibody detects Trefoil factor 1 protein at cytoplasm in mouse stomach by immunohistochemical analysis. Sample: Paraffin-embedded mouse stomach. Trefoil factor 1 antibody (GTX121461) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX121461 IHC-P Image
Immunohistochemical analysis of paraffin-embedded human gastric cancer, using Trefoil factor 1(GTX121461) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX121461 ICC/IF Image
Immunofluorescence analysis of methanol-fixed MCF-7, using Trefoil factor 1(GTX121461) antibody at 1:500 dilution.
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GTX121461 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with TFF1 antibody (GTX121461) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
