APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:1000 |
| 1:10-1:50 |
| 1:50-1:100 |
| 1:10-1:50 |
Not tested in other applications.
Calculated MW
Predict Reactivity
Rat(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.09% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
KLH conjugated synthetic peptide between 956-985 amino acids from the C-terminal region of human GTF2I.
Purification
Protein A purified, followed by peptide affinity purification.
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
general transcription factor IIi , BAP135 , BTKAP1 , DIWS , GTFII-I , IB291 , SPIN , TFII-I , WBS , WBSCR6
Cellular Localization
Cytoplasm,Nucleus
Background
This gene encodes a phosphoprotein containing six characteristic repeat motifs. The encoded protein binds to the initiator element (Inr) and E-box element in promoters and functions as a regulator of transcription. This locus, along with several other neighboring genes, is deleted in Williams-Beuren syndrome. There are many closely related genes and pseudogenes for this gene on chromosome 7. This gene also has pseudogenes on chromosomes 9, 13, and 21. Alternatively spliced transcript variants encoding multiple isoforms have been observed. [provided by RefSeq, Jul 2013]
Database
DATA IMAGES
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GTX81672 ICC/IF Image
ICC/IF analysis of A549 cells using GTX81672 TFII-I antibody, C-term.
Green : TFII-I
Red : Actin filament
Fixation : 4% paraformaldehyde (PFA) (20 min)
Permeabilization : Triton X-100 (0.1%, 10 min)
Dilution : 1:25
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GTX81672 WB Image
WB analysis of A431, HeLa cell using GTX81672 TFII-I antibody, C-term.
Loading : 35ug per lane
Dilution : 1:1000
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GTX81672 ICC/IF Image
ICC/IF analysis of HeLa cells using GTX81672 TFII-I antibody, C-term.
Green : TFII-I
Red : Actin filament
Blue : cell nucleus
Dilution : 1:25
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GTX81672 WB Image
WB analysis of HeLa cell lysate using GTX81672 TFII-I antibody, C-term.
Loading : 35ug
Dilution : 1:1000
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GTX81672 FACS Image
FACS analysis of K562 cells using GTX81672 TFII-I antibody, C-term.
Top histogram : negative control
Bottom histogram : K562 cells
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GTX81672 IHC-P Image
IHC-P analysis of human brain tissue using GTX81672 TFII-I antibody, C-term.
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REFERENCE
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REVIEW
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