APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500 - 1:2000 |
| 1:50 - 1:200 |
| 1:100 - 1:200 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS, 50% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
A synthetic peptide of human GTF2I
Purification
Purified by affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
general transcription factor IIi , BAP135 , BTKAP1 , DIWS , GTFII-I , IB291 , SPIN , TFII-I , WBS , WBSCR6
Cellular Localization
Cytoplasm,Nucleus
Background
This gene encodes a phosphoprotein containing six characteristic repeat motifs. The encoded protein binds to the initiator element (Inr) and E-box element in promoters and functions as a regulator of transcription. This locus, along with several other neighboring genes, is deleted in Williams-Beuren syndrome. There are many closely related genes and pseudogenes for this gene on chromosome 7. This gene also has pseudogenes on chromosomes 9, 13, and 21. Alternatively spliced transcript variants encoding multiple isoforms have been observed. [provided by RefSeq, Jul 2013]
Database
DATA IMAGES
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GTX65927 IHC-P Image
IHC-P analysis of human esophagus tissue using GTX65927 TFII-I antibody.
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GTX65927 IHC-P Image
IHC-P analysis of rat kidney tissue using GTX65927 TFII-I antibody.
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GTX65927 WB Image
WB analysis of mouse brain tissue lysate using GTX65927 TFII-I antibody. The signal was developed with ECL plus-Enhanced.
Dilution : 1:500
Loading : 25μg per lane
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GTX65927 IHC-P Image
IHC-P analysis of mouse liver tissue using GTX65927 TFII-I antibody.
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GTX65927 ICC/IF Image
ICC/IF analysis of A431 cells using GTX65927 TFII-I antibody.
Blue : DAPI for nuclear staining
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GTX65927 IHC-P Image
IHC-P analysis of rat brain tissue using GTX65927 TFII-I antibody.
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GTX65927 WB Image
WB analysis of BT-474 cell lysate using GTX65927 TFII-I antibody.
Dilution : 1:500
Loading : 25μg per lane
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REFERENCE
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REVIEW
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