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TFIIB antibody [1A4-A8-C3]

Anti-TFIIB antibody [1A4-A8-C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX49187
Anti-TFIIB antibody [1A4-A8-C3] used in IHC (Paraffin sections) (IHC-P). GTX49187
Anti-TFIIB antibody [1A4-A8-C3] used in Western Blot (WB). GTX49187

Cat No. GTX49187

Host

Mouse

Clonality

Monoclonal

Clone Name

1A4-A8-C3

Isotype

IgG2b

Application

WB, ICC/IF, IHC-P

Reactivity

Human
Package
100 μg ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

37 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.02% sodium azide, 50%,glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified recombinant human TFIIB protein fragments expressed in E.coli.

Purification

Affinity purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

General Transcription Factor Iib , Tf2B , Tfiib , Gtf2B

Cellular Localization

Nucleus

Background

GTF2B is one of the ubiquitous factors required for transcription initiation by RNA polymerase II. The protein localizes to the nucleus where it forms a complex (the DAB complex) with transcription factors IID and IIA. Transcription factor IIB serves as a bridge between IID, the factor which initially recognizes the promoter sequence, and RNA polymerase II.

Database

Research Area

DATA IMAGES

Anti-TFIIB antibody [1A4-A8-C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX49187

GTX49187 ICC/IF Image

ICC/IF analysis of HeLa cells using TFIIB antibody at a dilution of 1:50.

Anti-TFIIB antibody [1A4-A8-C3] used in IHC (Paraffin sections) (IHC-P). GTX49187

GTX49187 IHC-P Image

IHC-P analysis of human colon tissue using TFIIB antibody.

Anti-TFIIB antibody [1A4-A8-C3] used in Western Blot (WB). GTX49187

GTX49187 WB Image

WB analysis of Jurkat, MCF7 and HeLa lysates using TFIIB antibody at a dilution of 1:1,000.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 319