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TFIIB antibody [N1C3]

Comparison Orthogonal Overexpression
Anti-TFIIB antibody [N1C3] used in Western Blot (WB). GTX107327
Anti-TFIIB antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX107327
Anti-TFIIB antibody [N1C3] used in Western Blot (WB). GTX107327
Anti-TFIIB antibody [N1C3] used in Western Blot (WB). GTX107327
Anti-TFIIB antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107327
Anti-TFIIB antibody [N1C3] used in Western Blot (WB). GTX107327

Cat. No. GTX107327

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all TFIIB products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

35 kDa. ( Note )

Positive Control

HeLa nuclear extract , GTF2B-transfected 293T , 293T , A431 , HeLa , HepG2

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.56 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TFIIB. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950470

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

general transcription factor IIB , TF2B , TFIIB

Cellular Localization

Nucleus

Background

This gene encodes the general transcription factor IIB, one of the ubiquitous factors required for transcription initiation by RNA polymerase II. The protein localizes to the nucleus where it forms a complex (the DAB complex) with transcription factors IID and IIA. Transcription factor IIB serves as a bridge between IID, the factor which initially recognizes the promoter sequence, and RNA polymerase II. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-TFIIB antibody [N1C3] used in Western Blot (WB). GTX107327

GTX107327 WB Image

Comparison Orthogonal

HeLa whole cell and nuclear extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with TFIIB antibody [N1C3] (GTX107327) diluted at 1:500 and competitor's antibody (sc-274) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-TFIIB antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX107327

GTX107327 IHC-P Image

TFIIB antibody [N1C3] detects TFIIB protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
TFIIB stained by TFIIB antibody [N1C3] (GTX107327) diluted at 1:500.

Anti-TFIIB antibody [N1C3] used in Western Blot (WB). GTX107327

GTX107327 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with TFIIB antibody [N1C3] (GTX107327) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TFIIB antibody [N1C3] used in Western Blot (WB). GTX107327

GTX107327 WB Image

Comparison Orthogonal

HeLa whole cell and nuclear extracts (30 μg) were separated by 12% SDS-PAGE, and the membranes were blotted with TFIIB antibody [N1C3] (GTX107327) diluted at 1:1500 and competitor's antibody (sc-274) diluted at 1:100. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-TFIIB antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107327

GTX107327 ICC/IF Image

TFIIB antibody [N1C3] detects TFIIB protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TFIIB stained by TFIIB antibody [N1C3] (GTX107327) diluted at 1:1000.
Red: phalloidin, a cytoskeleton marker, diluted at 1:100.
Scale bar= 10μm.

Anti-TFIIB antibody [N1C3] used in Western Blot (WB). GTX107327

GTX107327 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with TFIIB antibody [N1C3] (GTX107327) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169