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TFIIB antibody

Anti-TFIIB antibody used in IHC (Paraffin sections) (IHC-P). GTX66408
Anti-TFIIB antibody used in IHC (Paraffin sections) (IHC-P). GTX66408
Anti-TFIIB antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX66408
Anti-TFIIB antibody used in IHC (Paraffin sections) (IHC-P). GTX66408
Anti-TFIIB antibody used in IHC (Paraffin sections) (IHC-P). GTX66408
Anti-TFIIB antibody used in Western Blot (WB). GTX66408

Cat. No. GTX66408

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all TFIIB products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

35 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 50-150 of human GTF2B (NP_001505.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

general transcription factor IIB , TF2B , TFIIB

Cellular Localization

Nucleus

Background

This gene encodes the general transcription factor IIB, one of the ubiquitous factors required for transcription initiation by RNA polymerase II. The protein localizes to the nucleus where it forms a complex (the DAB complex) with transcription factors IID and IIA. Transcription factor IIB serves as a bridge between IID, the factor which initially recognizes the promoter sequence, and RNA polymerase II. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-TFIIB antibody used in IHC (Paraffin sections) (IHC-P). GTX66408

GTX66408 IHC-P Image

IHC-P analysis of human esophageal tissue using GTX66408 TFIIB antibody.
Dilution : 1:100

Anti-TFIIB antibody used in IHC (Paraffin sections) (IHC-P). GTX66408

GTX66408 IHC-P Image

IHC-P analysis of mouse testis tissue using GTX66408 TFIIB antibody.
Dilution : 1:100

Anti-TFIIB antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX66408

GTX66408 ICC/IF Image

ICC/IF analysis of L929 cells using GTX66408 TFIIB antibody.
Blue : DAPI
Dilution : 1:100

Anti-TFIIB antibody used in IHC (Paraffin sections) (IHC-P). GTX66408

GTX66408 IHC-P Image

IHC-P analysis of human colon tissue using GTX66408 TFIIB antibody.
Dilution : 1:100

Anti-TFIIB antibody used in IHC (Paraffin sections) (IHC-P). GTX66408

GTX66408 IHC-P Image

IHC-P analysis of human lung cancer tissue using GTX66408 TFIIB antibody.
Dilution : 1:100

Anti-TFIIB antibody used in Western Blot (WB). GTX66408

GTX66408 WB Image

WB analysis of various sample lysates using GTX66408 TFIIB antibody. The signal was developed with ECL plus-Enhanced.
Dilution : 1:1000
Loading : 25μg per lane

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399