GeneTex
United States (US)

TFIIE beta antibody

GTX105029_40723_IP_19011506_466.jpg
GTX105029_40723_WB_19011506_425.jpg
GTX105029_40723_ChIP_19011506_684.jpg

Cat No. GTX105029

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IP, ChIP assay
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
IP 1:100-1:500
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

Positive Control

Jurkat nucleus

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine, Chicken, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TFIIE beta. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

FE antibody, TF2E2 antibody, TFIIE-B antibody, GTF2E2 antibody, TFIIE-beta antibody, transcription initiation factor IIE subunit beta antibody, general transcription factor IIE subunit 2 antibody, "general transcription factor IIE, polypeptide 2, beta 34kDa antibody"

Cellular Localization

Nucleus

Database

DATA IMAGES
GTX105029_40723_IP_19011506_466.jpg

GTX105029 IP Image

TFIIE beta antibody immunoprecipitates TFIIE beta protein in IP experiments. IP Sample: Jurkat whole cell lysate/extract A : 30 μg whole cell lysate/extract of TFIIE beta protein expressing Jurkat cells B : Control with 2.5 μg of pre-immune rabbit IgG C : Immunoprecipitation of TFIIE beta by 2.5 μg of TFIIE beta antibody (GTX105029) 12% SDS-PAGE The immunoprecipitated TFIIE beta protein was detected by TFIIE beta antibody (GTX105029) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.

GTX105029_40723_WB_19011506_425.jpg

GTX105029 WB Image

Sample (30 ug of whole cell lysate)
A: Jurkat nucleus
10% SDS PAGE
GTX105029 diluted at 1:5000

GTX105029_40723_ChIP_19011506_684.jpg

GTX105029 ChIP assay Image

Cross-linked ChIP was performed with HeLa chromatin extract and 5 μg of either control rabbit IgG or anti-TFIIE beta antibody. The precipitated DNA was detected by PCR with primer set targeting to GAPDH promoter or MyoD.

Reference
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169