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TFIIF antibody

KO/KD
Anti-TFIIF antibody used in Immunoprecipitation (IP). GTX114455
Anti-TFIIF antibody used in Western Blot (WB). GTX114455
Anti-TFIIF antibody used in Western Blot (WB). GTX114455
Anti-TFIIF antibody used in ChIP assay (ChIP assay). GTX114455

Cat. No. GTX114455

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IP, ChIP assay

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all TFIIF products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IP 1:100-1:500
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

58 kDa. ( Note )

Positive Control

293T , A431 , HeLa , NIH-3T3

Product Note

Knockdown/Knockout validation was supported by references (PMID:26990993)

Predict Reactivity

Rat, Bovine, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TFIIF. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11167162

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

general transcription factor IIF subunit 1 , BTF4 , RAP74 , TF2F1 , TFIIF

Cellular Localization

Nucleus

Background

TFIIF is a general transcription initiation factor that binds to RNA polymerase II and helps to recruit it to the initiation complex in collaboration with TFIIB. It promotes transcription elongation.

Database

Research Area

DATA IMAGES

Anti-TFIIF antibody used in Immunoprecipitation (IP). GTX114455

GTX114455 IP Image

TFIIF antibody immunoprecipitates TFIIF protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : 30 μg whole cell lysate/extract of TFIIF protein expressing 293T cells B : Control with 2.5 μg of pre-immune rabbit IgG C : Immunoprecipitation of TFIIF by 2.5 μg of TFIIF antibody (GTX114455) 7.5% SDS-PAGE The immunoprecipitated TFIIF protein was detected by TFIIF antibody (GTX114455) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.

Anti-TFIIF antibody used in Western Blot (WB). GTX114455

GTX114455 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
B: A431
C: HeLa
7.5% SDS PAGE
GTX114455 diluted at 1:3000

Anti-TFIIF antibody used in Western Blot (WB). GTX114455

GTX114455 WB Image

Sample (30 ug of whole cell lysate)
A: NIH-3T3
7.5% SDS PAGE
GTX114455 diluted at 1:3000

Anti-TFIIF antibody used in ChIP assay (ChIP assay). GTX114455

GTX114455 ChIP assay Image

Cross-linked ChIP was performed with HeLa chromatin extract and 5 μg of either control rabbit IgG or anti-TFIIF antibody. The precipitated DNA was detected by PCR with primer set targeting to GAPDH promoter or MyoD.

REFERENCE

Application Reference

Sundararaman B et al. Mol Cell 2016; 61 (6) : 903-13 Resources for the Comprehensive Discovery of Functional RNA Elements.
Application : WB, IP
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169