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TFPI antibody, C-term

Overexpression
Anti-TFPI antibody, C-term used in Western Blot (WB). GTX89985
Anti-TFPI antibody, C-term used in Western Blot (WB). GTX89985

Cat. No. GTX89985

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human
Package
100 μg ($399)
See all TFPI products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.3-1μg/ml
Not tested in other applications.

Calculated MW

35 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX89985-PEP).

Product Note

This antibody is expected to recognize isoform a (NP_006278.1) only.

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-VKIAYEEIFVKNM, from the C Terminus of the protein sequence according to NP_006278.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

tissue factor pathway inhibitor , EPI , LACI , TFI , TFPI1

Background

This gene encodes a Kunitz-type serine protease inhibitor that regulates the tissue factor (TF)-dependent pathway of blood coagulation. The coagulation process initiates with the formation of a factor VIIa-TF complex, which proteolytically activates additional proteases (factors IX and X) and ultimately leads to the formation of a fibrin clot. The product of this gene inhibits the activated factor X and VIIa-TF proteases in an autoregulatory loop. Inhibition of the encoded protein restores hemostasis in animal models of hemophilia. This gene encodes multiple protein isoforms that differ in their inhibitory activity, specificity and cellular localization. [provided by RefSeq, Jul 2016]

Database

Research Area

DATA IMAGES

Anti-TFPI antibody, C-term used in Western Blot (WB). GTX89985

GTX89985 WB Image

Overexpression

WB analysis of HEK293 overexpressing TFPI (mock transfection in first lane) using GTX89985 TFPI antibody, C-term.

Anti-TFPI antibody, C-term used in Western Blot (WB). GTX89985

GTX89985 WB Image

WB analysis of HeLa lysate using GTX89985 TFPI antibody, C-term.
Dilution : 0.3μg/ml
Loading : 35μg protein in RIPA buffer

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399