Sample (30 ug of whole cell lysate)
A: K562
B: HL-60
7.5% SDS PAGE
GTX110441 diluted at 1:1000

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TFR2 antibody [N3C2], Internal (GTX110441) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

TFR2 antibody [N3C2], Internal detects TFR2 protein at cytosol on human hepatoma by immunohistochemical analysis.
Sample: Paraffin-embedded hepatoma.
TFR2 antibody [N3C2], Internal (GTX110441) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min