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TFR2 antibody [N3C2], Internal

Orthogonal
Anti-TFR2 antibody [N3C2], Internal used in Western Blot (WB). GTX110441
Anti-TFR2 antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX110441
Anti-TFR2 antibody [N3C2], Internal used in Western Blot (WB). GTX110441

Cat No. GTX110441

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($329),
25 μl ($169)
See all TFR2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

89 kDa. ( Note )

Positive Control

K562 , HL-60

Predict Reactivity

Mouse, Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TFR2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11165062

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

transferrin receptor 2 , HFE3 , TFRC2

Cellular Localization

Cell membrane; Single-pass type II membrane protein , Isoform Beta: Cytoplasm (Probable)

Background

This gene is a member of the transferrin receptor-like family and encodes a single-pass type II membrane protein with a protease associated (PA) domain, an M28 peptidase domain and a transferrin receptor-like dimerization domain. This protein mediates cellular uptake of transferrin-bound iron and mutations in this gene have been associated with hereditary hemochromatosis type III. Alternatively spliced variants which encode different protein isoforms have been described; however, not all variants have been fully characterized. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-TFR2 antibody [N3C2], Internal used in Western Blot (WB). GTX110441

GTX110441 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TFR2 antibody [N3C2], Internal (GTX110441) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
The observed M.W. is based on the publication: PMID: 19254567

Anti-TFR2 antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX110441

GTX110441 IHC-P Image

TFR2 antibody [N3C2], Internal detects TFR2 protein at cytosol on human hepatoma by immunohistochemical analysis.
Sample: Paraffin-embedded hepatoma.
TFR2 antibody [N3C2], Internal (GTX110441) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-TFR2 antibody [N3C2], Internal used in Western Blot (WB). GTX110441

GTX110441 WB Image

Sample (30 ug of whole cell lysate)
A: K562
B: HL-60
7.5% SDS PAGE
GTX110441 diluted at 1:1000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169