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TGF alpha antibody [MF9]

Anti-TGF alpha antibody [MF9] used in IHC (Paraffin sections) (IHC-P). GTX35102

Cat. No. GTX35102

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 MF9

Isotype

 IgG1

Application

 ICC/IF, IHC-P, ELISA

Reactivity

 Human, Mouse, Rat, Bovine
Package
100 μg ($529)
See all TGF alpha products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF 1-2μg/ml
IHC-P 2-4μg/ml for 30 min at RT
ELISA 2-4μg/ml (for coating)

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.

ELISA
For ELISA coating, recommend using BSA-free format (please contact us for PBS only format).
Not tested in other applications.

Calculated MW

17 kDa. ( Note )

Product Note

This antibody reacts with the C-terminus of TGF alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. The staining with Ab-1 is completely blocked by the peptide used for raising this antibody.

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

A 17-amino acid synthetic peptide from the carboxyl-terminus of rat TGFα (exact sequence is proprietary)

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Cellular Localization

Secreted,Extracellular space,Cell membrane

Background

acts as an EGF receptor ligand; inhibits apoptosis that results from epithelial detachment from the extracellular matrix [RGD, Feb 2006]

Database

Research Area

DATA IMAGES

Anti-TGF alpha antibody [MF9] used in IHC (Paraffin sections) (IHC-P). GTX35102

GTX35102 IHC-P Image

IHC-P analysis of human testicular carcinoma tissue using GTX35102 TGF alpha antibody [MF9].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 529