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TGF alpha antibody [P/T1]

Anti-TGF alpha antibody [P/T1] used in IHC (Paraffin sections) (IHC-P). GTX35106

Cat. No. GTX35106

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 P/T1

Isotype

 IgG1

Application

 ICC/IF, IHC-P, FACS

Reactivity

 Human, Zebrafish, Rabbit
Package
100 μg ($529)
See all TGF alpha products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF 1-2μg/ml
IHC-P 2-4μg/ml for 30 min at RT
FACS 1-2μg/10⁶ cells

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
Not tested in other applications.

Calculated MW

17 kDa. ( Note )

Product Note

This antibody reacts with the TGF alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. The staining with this MAb is completely blocked by the peptide used for raising this antibody.

PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A 10-amino acid synthetic peptide (aa34-43; PPVAAAVVSH) from human TGFα.

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

TFGA , TGFA , transforming growth factor alpha

Cellular Localization

Secreted,Extracellular space,Cell membrane

Background

This gene encodes a growth factor that is a ligand for the epidermal growth factor receptor, which activates a signaling pathway for cell proliferation, differentiation and development. This protein may act as either a transmembrane-bound ligand or a soluble ligand. This gene has been associated with many types of cancers, and it may also be involved in some cases of cleft lip/palate. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]

Database

Research Area

DATA IMAGES

Anti-TGF alpha antibody [P/T1] used in IHC (Paraffin sections) (IHC-P). GTX35106

GTX35106 IHC-P Image

IHC-P analysis of human testicular carcinoma tissue using GTX35106 TGF alpha antibody [P/T1].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 529