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TGF alpha antibody

Anti-TGF alpha antibody used in Western Blot (WB). GTX129103
Anti-TGF alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX129103

Cat No. GTX129103

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

17 kDa. ( Note )

Positive Control

TGFA-transfected 293T

Predict Reactivity

Mouse, Rat, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human TGF alpha. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

transforming growth factor alpha , TFGA

Cellular Localization

Transforming growth factor alpha: Secreted , extracellular space , Protransforming growth factor alpha: Cell membrane; Single-pass type I membrane protein

Background

Transforming growth factors (TGFs) are biologically active polypeptides that reversibly confer the transformed phenotype on cultured cells. TGF-alpha shows about 40% sequence homology with epidermal growth factor (EGF; MIM 131530) and competes with EGF for binding to the EGF receptor (MIM 131550), stimulating its phosphorylation and producing a mitogenic response.[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-TGF alpha antibody used in Western Blot (WB). GTX129103

GTX129103 WB Image

TGF alpha antibody detects TGF alpha protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg whole cell lysate/extract of TGFA-transfected 293T cells
15 % SDS-PAGE
TGF alpha antibody (GTX129103) dilution: 1:1000

Anti-TGF alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX129103

GTX129103 IHC-P Image

TGF alpha antibody detects TGF alpha protein at membrane on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
TGF alpha antibody (GTX129103) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf