GeneTex
United States (US)

TGF alpha antibody

TGF-alpha-antibody-GTX15541-IHC-1_18121411_975.jpg
TGF-alpha-antibody-GTX15541-IHC-2_18121411_890.jpg

Cat No. GTX15541

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application IHC-P, IHC
Reactivity Human
APPLICATION

Application Note

IHC-P: Use at a dilution of 1/50 for 10 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

17 kDa. ( Note )

Positive Control

Normal colon and anterior pituitary
PROPERTIES

Form

Liquid

Buffer

Phosphate-buffered saline, pH 7.4 containing 1% BSA and 0.09% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Antigen Species

Human

Immunogen

A synthetic peptide derived from near C-terminal of human TGF alpha.

Purification

Immunogen affinity purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Transforming Growth Factor Alpha,Tfga,Tgfa

Cellular Localization

Type I membrane protein (precursor form) and extracellular (mature form).

Background

Transforming growth factor-alpha (TGF alpha) is an acid- and heat-stable 50 amino acid protein of 5,500 MW originally discovered in rodents and humans. TGF alpha protien is 33% homologous to epidermal growth factor (EGF). TGF alpha binds to the EGF receptor, mediating tyrosine phosphorylation of the receptor, and promotes anchorage-independent growth of normal rat fibroblasts in soft agar in the presence of transforming growth factor-beta. TGF alpha is secreted by a variety of transformed cells and tumors, embryonic cells and some normal adult cells . TGF alpha bioactivity has also been found in the urine of cancer patients. It has been suggested that it may act as an autocrine growth factor for the induction or maintenance of malignancy.

Database

Research Area

DATA IMAGES
TGF-alpha-antibody-GTX15541-IHC-1_18121411_975.jpg

GTX15541 IHC Image


TGF-alpha-antibody-GTX15541-IHC-2_18121411_890.jpg

GTX15541 IHC Image

Human pituitary stained with GTX15541