IHC-P: Use at a dilution of 1/50 for 10 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. Optimal dilutions/concentrations should be determined by the end user.
Normal colon and anterior pituitary
Phosphate-buffered saline, pH 7.4 containing 1% BSA and 0.09% sodium azide
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.
A synthetic peptide derived from near C-terminal of human TGF alpha.
Immunogen affinity purified
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Transforming Growth Factor Alpha,Tfga,Tgfa
Type I membrane protein (precursor form) and extracellular (mature form).
Transforming growth factor-alpha (TGF alpha) is an acid- and heat-stable 50 amino acid protein of 5,500 MW originally discovered in rodents and humans. TGF alpha protien is 33% homologous to epidermal growth factor (EGF). TGF alpha binds to the EGF receptor, mediating tyrosine phosphorylation of the receptor, and promotes anchorage-independent growth of normal rat fibroblasts in soft agar in the presence of transforming growth factor-beta. TGF alpha is secreted by a variety of transformed cells and tumors, embryonic cells and some normal adult cells . TGF alpha bioactivity has also been found in the urine of cancer patients. It has been suggested that it may act as an autocrine growth factor for the induction or maintenance of malignancy.