GeneTex

  • Country / Location Selection

United States (US)

TGF alpha antibody

Anti-TGF alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX27293
Anti-TGF alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX27293

Cat. No. GTX27293

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ELISA, Neutralizing/Inhibition

Reactivity

 Human
Package
25 μg ($399)
See all TGF alpha products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.1 - 0.2 μg/mL
IHC-P Assay dependent
ELISA 0.5 μg/mL
Neutralizing/Inhibition 0.1 - 0.15 μg/mL

Note :

Neutralizing/Inhibition
To yield one-half maximal inhibition [ND₅₀] of the biological activity of hTGF-alpha (2 ng/mL)
Not tested in other applications.

Calculated MW

17 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hTGF-alpha (human Transforming Growth Factor-alpha).

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

transforming growth factor alpha , TFGA

Background

Transforming Growth Factor-alpha (TGFa), originally discovered in 1978 in conditioned medium of retrovirus-transformed fibroblasts (ref 1), is a protein that reversibly confers a transformed phenotype upon normal non-neoplastic cells, such as normal rat kidney (NRK) fibroblasts (ref 2). The transforming activity of TGFa was later shown to require the presence of Transforming Growth Factor-beta, which potentiates the action of TGFa via a separate receptor (ref 2-4).

Database

Research Area

DATA IMAGES

Anti-TGF alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX27293

GTX27293 IHC-P Image

IHC-P analysis of human breast invasive ductal carcinoma tissue using GTX27293 TGF alpha antibody.
Working concentration : 0.5 μg/ml
Antigen retrieval : Heat induced antigen retrieval with a pH 6.0 Sodium Citrate buffer.

Anti-TGF alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX27293

GTX27293 IHC-P Image

IHC-P analysis of human breast invasive ductal carcinoma tissue using GTX27293 TGF alpha antibody.
Working concentration : 0.5 μg/ml
Antigen retrieval : Heat induced antigen retrieval with a pH 6.0 Sodium Citrate buffer.

REFERENCE

There are currently no references for TGF alpha antibody (GTX27293). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for TGF alpha antibody (GTX27293). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Package List Price ($)
$ 399