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TGF beta 1 antibody [9016.2]

Cat No. GTX10517

Host Mouse
Clonality Monoclonal
Clone Name 9016.2
Isotype IgG1
Application WB, ICC/IF, FACS, ELISA, IHC, Neutralizing/Blocking
Reactivity Human, Mouse, Rat
Package
100 μg ($319)
See all TGF beta 1 products
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1 ug/mL (Under non-reducing conditions only)
ICC/IF Assay dependent
FACS 2.5 μg/10⁶ cells
ELISA capture 2-8 μg/mL or detection 0.1-0.4 μg/mL
IHC 3-25 μg/mL
Neutralizing/Blocking 0.3-1.0 μg/mL

Note :

Neutralizing/Blocking
Measured by its ability to neutralize TGF­β1 inhibition of IL­4­ dependent proliferation in the HT­2 mouse T cell line.
Not tested in other applications.

Calculated MW

44 kDa. ( Note )

Specificity/Sensitivity

By capture ELISA, <2% cross-reactivity is observed with TGF-β3 and TGF-β5.
PROPERTIES

Form

Liquid

Buffer

PBS containing carbohydrates

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

purified, CHO cell-derived, recombinant TGF-β1 and latent TGF-β1.

Purification

Protein A/G purified
From tissue culture supernatant

Endotoxin

Not determined

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

transforming growth factor beta 1 , CED , DPD1 , IBDIMDE , LAP , TGFB , TGFbeta

Background

Transforming Growth Factor Beta 1 is a multifunctional peptide capable of influencing cell proliferation, differentiation, and other functions in a wide range of cell types. Transformed as well as non-neoplastic tissues, release transforming growth factors; and essentially all cells possess a specific TGF beta 1 receptor. The multi-modal nature of TGF beta 1 is seen in its ability to stimulate or inhibit cellular proliferation. In general, cells of mesenchymal origin appear to be stimulated by TGF beta 1 whereas hepatocytes, T and B lymphocytes, keratinocytes and many epithelial cells are inhibited by the peptide. TGF beta 1 interacts with Epidermal Growth Factor, Platelet Derived Growth Factor, Fibroblast Growth Factor, and IL2 either by enhancing or antagonizing their characteristic actions. TGF beta 1 plays a fundamental role in tissue growth and differentiation involvement in adipogenesis, myogenesis, chondrogenesis, osteogenesis, epithelial cell differentiation and immune cell function.

Database

Research Area

REFERENCE
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Package List Price ($)
$ 319
Currently in production