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TGF beta 1 antibody

Comparison Orthogonal Overexpression
Anti-TGF beta 1 antibody used in Western Blot (WB). GTX130023
Anti-TGF beta 1 antibody used in Western Blot (WB). GTX130023
Anti-TGF beta 1 antibody used in Western Blot (WB). GTX130023
Anti-TGF beta 1 antibody used in Western Blot (WB). GTX130023

Cat. No. GTX130023

Reference 16
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all TGF beta 1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

44 kDa. ( Note )

Positive Control

K562 , THP-1 , HL-60 , TGFB1-tranfected 293T

Predict Reactivity

Sheep, Bovine, Chicken, Guinea pig, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.23 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human TGF beta 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886158

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

transforming growth factor beta 1 , CED , DPD1 , IBDIMDE , LAP , TGFB , TGFbeta

Cellular Localization

Secreted

Background

TGFB is a multifunctional peptide that controls proliferation, differentiation, and other functions in many cell types. TGFB acts synergistically with TGFA (MIM 190170) in inducing transformation. It also acts as a negative autocrine growth factor. Dysregulation of TGFB activation and signaling may result in apoptosis. Many cells synthesize TGFB and almost all of them have specific receptors for this peptide. TGFB1, TGFB2 (MIM 190220), and TGFB3 (MIM 190230) all function through the same receptor signaling systems.[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-TGF beta 1 antibody used in Western Blot (WB). GTX130023

GTX130023 WB Image

Comparison

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with TGF beta 1 antibody (GTX130023) diluted at 1:500 and competitor's antibody (CST#3709) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-TGF beta 1 antibody used in Western Blot (WB). GTX130023

GTX130023 WB Image

Comparison Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with TGF beta 1 antibody (GTX130023) diluted at 1:500 and competitor's antibody (#3709) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-TGF beta 1 antibody used in Western Blot (WB). GTX130023

GTX130023 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with TGF beta 1 antibody (GTX130023) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TGF beta 1 antibody used in Western Blot (WB). GTX130023

GTX130023 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TGF beta 1 antibody (GTX130023) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE

Application Reference

Chiou WC et al. Biomed Pharmacother 2023; 161 : 114481 Ovatodiolide inhibits SARS-CoV-2 replication and ameliorates pulmonary fibrosis through suppression of the TGF-β/TβRs signaling pathway.
Application : IHC-P
Reactivity : Mouse
Mang Y et al. Ann Transl Med 2022; 10 (19) : 1066 Characteristics of pre-sensitization-related acute antibody-mediated rejection in a rat model of orthotopic liver transplantation.
Application : WB
Reactivity : Rat
Peng-Hsiang Fang et al. Mol Med 2022; 28 (1) : 71 Cobalt protoporphyrin promotes human keratinocyte migration under hyperglycemic conditions.
Application : WB
Reactivity : Human
Shannon Cunningham et al. Cancers (Basel) 2021; 13 (5) FLASH Proton Pencil Beam Scanning Irradiation Minimizes Radiation-Induced Leg Contracture and Skin Toxicity in Mice.
Application : WB
Reactivity : Mouse
Cunningham S. et al. Cancers 2021; 13 (5) : 1012 FLASH Proton Pencil Beam Scanning Irradiation Minimizes Radiation-Induced Leg Contracture and Skin Toxicity in Mice.
Application : WB
Reactivity : Mouse
Show More +
Submit a Reference

REVIEW

TGF beta 1 antibody Cat. No. GTX130023

Rating
( Average 5 based on 1 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Anti-TGF beta 1 antibody used in Western Blot (WB). GTX130023
Date : Anonymous submitted on 11-May-2023
Score :
Application Tested : WB
Sample Species : Hu
Sample : MDA-MB-231 cells or conditional medium with/without siHSP70 treatment
Amount used : 10μg
Blocking : 5% skim milk in TBS-T, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 15Hr
Submit a Review
Application Reference
Chiou WC et al. Biomed Pharmacother 2023; 161:114481 Ovatodiolide inhibits SARS-CoV-2 replication and ameliorates pulmonary fibrosis through suppression of the TGF-β/TβRs signaling pathway.
Application : IHC-P
Reactivity : Mouse
Mang Y et al. Ann Transl Med 2022; 10 (19):1066 Characteristics of pre-sensitization-related acute antibody-mediated rejection in a rat model of orthotopic liver transplantation.
Application : WB
Reactivity : Rat
Peng-Hsiang Fang et al. Mol Med 2022; 28 (1):71 Cobalt protoporphyrin promotes human keratinocyte migration under hyperglycemic conditions.
Application : WB
Reactivity : Human
Shannon Cunningham et al. Cancers (Basel) 2021; 13 (5) FLASH Proton Pencil Beam Scanning Irradiation Minimizes Radiation-Induced Leg Contracture and Skin Toxicity in Mice.
Application : WB
Reactivity : Mouse
Cunningham S. et al. Cancers 2021; 13 (5):1012 FLASH Proton Pencil Beam Scanning Irradiation Minimizes Radiation-Induced Leg Contracture and Skin Toxicity in Mice.
Application : WB
Reactivity : Mouse
Mazumdar A et al. Int J Mol Sci 2020; 21 (15) Osteosarcoma-Derived Extracellular Vesicles Induce Lung Fibroblast Reprogramming.
Ho KH et al. Cell Mol Neurobiol 2020; miR-4286 is Involved in Connections Between IGF-1 and TGF-β Signaling for the Mesenchymal Transition and Invasion by Glioblastomas.
Application : WB
Reactivity : Human
Chen HY et al. Chem Biol Interact 2020; 331:109249 TGF-β1 signaling protects retinal ganglion cells from oxidative stress via modulation of the HO-1/Nrf2 pathway.
Application : WB
Reactivity : Mouse
Dantas WS et al. Diabetes 2020; 69 (8):1675-1691 Exercise-Induced Increases in Insulin Sensitivity After Bariatric Surgery Are Mediated By Muscle Extracellular Matrix Remodeling.
Application : WB
Reactivity : Human
Ho YJ et al. Theranostics 2019; 9 (24):7370-7383 Normalization of Tumor Vasculature by Oxygen Microbubbles with Ultrasound.
Application : WB
Reactivity : Mouse
Park M et al. Stem Cell Res Ther 2019; 10 (1):246 Human placenta-derived mesenchymal stem cells ameliorate orbital adipogenesis in female mice models of Graves' ophthalmopathy.
Application : WB
Reactivity : Human
Gu J et al. Evid Based Complement Alternat Med 2019; 2019:1358469 Huangqi Shengmai Yin Protects against Radiation-Induced Cardiac Fibrosis Injury by Regulating the TGF-帣1/Smads and MMPs.
Application : WB, IHC-P
Reactivity : Rat
Yamaguchi T et al. J Pharmacol Sci 2019; 139 (2):112-119 A dipeptidyl peptidase-4 (DPP-4) inhibitor, linagliptin, attenuates cardiac dysfunction after myocardial infarction independently of DPP-4.
Application : WB
Reactivity : Rat
Wu CY et al. PLoS One 2019; 14 (3):e0213400 A potential new approach for treating systemic sclerosis: Dedifferentiation of SSc fibroblasts and change in the microenvironment by blocking store-operated Ca2+ entry.
Application : WB
Deng S et al. J * Cytokine Res 2019; 39 (2):106-116 Transforming Growth Factor-β-Neutralizing Antibodies Improve Alveolarization in the Oxygen-Exposed Newborn Mouse Lung.
Application : WB, IHC-P
Reactivity : Mouse
Zhou Y et al. Cancer Med 2018; (Epub) Iron overloaded polarizes macrophage to proinflammation phenotype through ROS/acetyl-p53 pathway.
Application : WB
Reactivity : Mouse
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169