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TGF beta 1 antibody

Anti-TGF beta 1 antibody used in Western Blot (WB). GTX130023
Anti-TGF beta 1 antibody used in Western Blot (WB). GTX130023
Anti-TGF beta 1 antibody used in Western Blot (WB). GTX130023

Cat No. GTX130023

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

44 kDa. ( Note )

Positive Control

K562 , THP-1 , HL-60 , TGFB1-tranfected 293T

Predict Reactivity

Sheep, Bovine, Dog, Chicken, Guinea pig, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.46 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of Human TGF beta 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Transforming Growth Factor Beta 1,Ced,Dpd1,Lap,Tgfb,Tgfbeta,Tgfb1

Cellular Localization

Secreted

Background

TGFB is a multifunctional peptide that controls proliferation, differentiation, and other functions in many cell types. TGFB acts synergistically with TGFA (MIM 190170) in inducing transformation. It also acts as a negative autocrine growth factor. Dysregulation of TGFB activation and signaling may result in apoptosis. Many cells synthesize TGFB and almost all of them have specific receptors for this peptide. TGFB1, TGFB2 (MIM 190220), and TGFB3 (MIM 190230) all function through the same receptor signaling systems.[supplied by OMIM]

Database

Research Area

DATA IMAGES
Anti-TGF beta 1 antibody used in Western Blot (WB). GTX130023

GTX130023 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membranes were blotted with TGF beta 1 antibody (GTX130023) diluted at 1:500 and competitor's antibody (CST#3709) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-TGF beta 1 antibody used in Western Blot (WB). GTX130023

GTX130023 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membranes were blotted with TGF beta 1 antibody (GTX130023) diluted at 1:500 and competitor's antibody (#3709) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-TGF beta 1 antibody used in Western Blot (WB). GTX130023

GTX130023 WB Image

Whole cell extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with TGF beta 1 antibody (GTX130023) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169