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TGF beta 1 antibody

Anti-TGF beta 1 antibody used in Western Blot (WB). GTX45121
Anti-TGF beta 1 antibody used in Western Blot (WB). GTX45121
Anti-TGF beta 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX45121

Cat. No. GTX45121

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P, IHC-Fr, IHC

Reactivity

Human, Mouse, Rat
Package
100 μg ($399)

APPLICATION

Application Note

Recommended Starting Dilutions:
For WB: WB: 1:1000-1:10000.
For IHC: Use at a dilution of 1:200 - 1:500.
Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

44 kDa. ( Note )

Positive Control

TGFB1-transfected 293T

Product Note

TGFB1 detected in RAW264.7 cells

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.1% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Pig

Immunogen

Recombinant protein - pig TGFB1

Purification

Protein A affinity purified
From polyclonal serum

Conjugation

Unconjugated

RRID

AB_1080946

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Database

Research Area

DATA IMAGES

Anti-TGF beta 1 antibody used in Western Blot (WB). GTX45121

GTX45121 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TGF beta 1 antibody (GTX45121) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TGF beta 1 antibody used in Western Blot (WB). GTX45121

GTX45121 WB Image

TGF beta1 detected by western blot in RAW264.7 cells using anti-TGFB1 antibody (GTX45121)

Anti-TGF beta 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX45121

GTX45121 IHC-P Image

The data was published in the 2022 in Toxics. PMID: 35622687

REFERENCE

REVIEW

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Application Reference
SDS
Sodium Azide.pdf
Package List Price ($)
$ 399