GeneTex
United States (US)

TGF beta 2 antibody [220ct16.4.3.1]

GTX16335_1369_WB_18121410_915.jpg
GTX16335_1368_WB_18121410_331.jpg
GTX16335_654_IHC-P_18121410_314.jpg
GTX16335_655_IHC-P_18121410_104.jpg
GTX16335_554_ICC-IF_18121410_168.jpg

Cat No. GTX16335

Host Mouse
Clonality Monoclonal
Clone Name 220ct16.4.3.1
Isotype IgG1
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:100-1:1000
ICC/IF 1:10-1:50
IHC-P 1:10-1:50
Not tested in other applications.

Calculated MW

48 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS, 0.09% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

This antibody is generated from mouse immunized with TGFB2 recombinant protein.

Purification

Protein G purified, followed by dialysis against PBS.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Transforming Growth Factor Beta 2,G-Tsf,Lds4,Tgf-Beta2,Tgfb2

Background

This gene encodes a member of the transforming growth factor beta (TGFB) family of cytokines, which are multifunctional peptides that regulate proliferation, differentiation, adhesion, migration, and other functions in many cell types by transducing their signal through combinations of transmembrane type I and type II receptors (TGFBR1 and TGFBR2) and their downstream effectors, the SMAD proteins. Disruption of the TGFB/SMAD pathway has been implicated in a variety of human cancers. The encoded protein is secreted and has suppressive effects of interleukin-2 dependent T-cell growth. Translocation t(1;7)(q41;p21) between this gene and HDAC9 is associated with Peters' anomaly, a congenital defect of the anterior chamber of the eye. The knockout mice lacking this gene show perinatal mortality and a wide range of developmental, including cardiac, defects. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Sep 2010]

Database

Research Area

DATA IMAGES
GTX16335_1369_WB_18121410_915.jpg

GTX16335 WB Image

WB analysis of A549 cell lysate (35μg/lane) using GTX16335 TGF beta 2 antibody [220ct16.4.3.1].

GTX16335_1368_WB_18121410_331.jpg

GTX16335 WB Image

WB analysis of various samples using GTX16335 TGF beta 2 antibody [220ct16.4.3.1].
Lane 1: 293 whole cell lysate
Lane 2: K562 whole cell lysate
Loading : 20 µg per lane
Dilution : 1:500-1:1000

GTX16335_654_IHC-P_18121410_314.jpg

GTX16335 IHC-P Image

IHC-P analysis of human breast carcinoma using GTX16335 TGF beta 2 antibody [220ct16.4.3.1].

GTX16335_655_IHC-P_18121410_104.jpg

GTX16335 IHC-P Image

IHC-P analysis of human colon carcinoma using GTX16335 TGF beta 2 antibody [220ct16.4.3.1].

GTX16335_554_ICC-IF_18121410_168.jpg

GTX16335 ICC/IF Image

ICC/IF analysis of A549 cells using GTX16335 TGF beta 2 antibody [220ct16.4.3.1].
Green : TGF beta 2
Blue : DAPI