GeneTex
United States (US)

TGF beta 2 antibody

Cat No. GTX10850

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, ELISA, Neut
Reactivity Human, Pig
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.1-0.2 μg/ml
IHC-P Assay dependent
ELISA Assay dependent
Neut Assay dependent
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Specificity/Sensitivity

The antibody recognizes TGF-β2 and TGF-β1.2. By immunoblotting (non-reducing conditions) and ELISA, the antibody shows less than 2% cross-reactivity with TGF-β1, TGF-β3, and TGF-β5.
PROPERTIES

Form

Liquid

Buffer

PBS containing carbohydrates

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Pig

Immunogen

porcine transforming growth factor-β2.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Transforming Growth Factor Beta 2,G-Tsf,Lds4,Tgf-Beta2,Tgfb2

Background

Transforming Growth Factor beta 2 (TGF beta 2) is a member of the TGF beta family of growth factors. The TGF beta polypeptides are multifunctional; capable of influencing cell proliferation, differentiation, and other functions in a wide range of cell types. Transformed, as well as nonneoplastic tissues, release transforming growth factors; and essentially all mammalian cells possess a specific TGF receptor. The multi-modal nature of TGF beta is seen in its ability to stimulate or inhibit cellular proliferation. In general, cells of mesenchymal origin appear to be stimulated by TGF beta whereas cells of epithelial or neuroectodermal origin are inhibited by the peptide. TGF beta 1, TGF beta 2, and TGF beta 1.2 appear to be equivalent in biological activity, although there does appear to be differences in binding to certain types of receptors. TGF beta 2 is produced by many cell types and has been found in the highest concentration in porcine platelets and mammalian bone. Latent TGF beta 2 is the prominent isoform found in body fluids such as amniotic fluid, breast milk, and the aqueous and vitreous humor of the eye.

Database

Research Area