APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500 - 1:2000 |
| 1:100-1:500 |
| 1:50 - 1:200 |
Not tested in other applications.
Calculated MW
Predict Reactivity
Rabbit, Sheep, Bovine, Dog, Pig, Xenopus(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 150mM NaCl, 50% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
A synthesized peptide derived from human TGF beta Receptor I , corresponding to a region within the internal amino acids.
Purification
Purified by antigen-affinity chromatography
From serum
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
AAT5 , ACVRLK4 , ALK5 , ESS1 , LDS1 , LDS1A , LDS2A , MSSE , SKR4 , TGFBR1 , TGFR1 , tbetaRI , transforming growth factor beta receptor 1 , TGF beta Receptor 1 , TGF beta Receptor I , TGF-beta receptor type-1
Cellular Localization
Cell membrane,Cell junction,Tight junction
Background
The protein encoded by this gene forms a heteromeric complex with type II TGF-beta receptors when bound to TGF-beta, transducing the TGF-beta signal from the cell surface to the cytoplasm. The encoded protein is a serine/threonine protein kinase. Mutations in this gene have been associated with Loeys-Dietz aortic aneurysm syndrome (LDAS). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]
Database
Research Area
DATA IMAGES
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GTX00840 IHC-P Image
IHC-P analysis of human colorectal cancer using GTX00840 TGF beta Receptor I antibody. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100
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GTX00840 IHC-P Image
IHC-P analysis of mouse ovary tissue using GTX00840 TGF beta Receptor I antibody. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100
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GTX00840 IHC-P Image
IHC-P analysis of rat brain tissue using GTX00840 TGF beta Receptor I antibody. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100
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GTX00840 WB Image
WB analysis of HepG2 cell lysate using GTX00840 TGF beta Receptor I antibody.
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GTX00840 IHC-P Image
IHC-P analysis of human normal tissues adjacent to esophageal cancer using GTX00840 TGF beta Receptor I antibody. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100
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GTX00840 IHC-P Image
IHC-P analysis of human mammary cancer using GTX00840 TGF beta Receptor I antibody. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100
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GTX00840 IHC-P Image
IHC-P analysis of rat lung tissue using GTX00840 TGF beta Receptor I antibody. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100
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GTX00840 IHC-P Image
IHC-P analysis of mouse heart tissue using GTX00840 TGF beta Receptor I antibody. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100
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GTX00840 IHC-P Image
IHC-P analysis of human normal tissues adjacent to lung cancer using GTX00840 TGF beta Receptor I antibody. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100
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GTX00840 IHC-P Image
IHC-P analysis of mouse kidney tissue using GTX00840 TGF beta Receptor I antibody. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100
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GTX00840 ICC/IF Image
ICC/IF analysis of PFA-fixed LoVo cells using GTX00840 TGF beta Receptor I antibody.
Permeabilization : 0.1% saponin prior to blocking in 10% serum for 45 minutes at 37ºC
Dilution : 1:400
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GTX00840 IHC-P Image
IHC-P analysis of human pancreatic cancer using GTX00840 TGF beta Receptor I antibody. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100
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GTX00840 IHC-P Image
IHC-P analysis of human colorectal cancer using GTX00840 TGF beta Receptor I antibody. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100
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GTX00840 IHC-P Image
IHC-P analysis of human gastric cancer using GTX00840 TGF beta Receptor I antibody. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100
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GTX00840 IHC-P Image
IHC-P analysis of mouse spleen tissue using GTX00840 TGF beta Receptor I antibody. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100
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GTX00840 IHC-P Image
IHC-P analysis of mouse stomach tissue using GTX00840 TGF beta Receptor I antibody. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100
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GTX00840 IHC-P Image
IHC-P analysis of human normal tissues adjacent to liver cancer using GTX00840 TGF beta Receptor I antibody. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100
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GTX00840 IHC-P Image
IHC-P analysis of rat stomach tissue using GTX00840 TGF beta Receptor I antibody. Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100
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GTX00840 IHC-P Image
IHC-P analysis of human normal tissues adjacent to pancreatic cancer using GTX00840 TGF beta Receptor I antibody. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100
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GTX00840 IHC-P Image
IHC-P analysis of mouse brain tissue using GTX00840 TGF beta Receptor I antibody. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100
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GTX00840 IHC-P Image
IHC-P analysis of rat ovary tissue using GTX00840 TGF beta Receptor I antibody. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100
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GTX00840 IHC-P Image
IHC-P analysis of human esophageal cancer using GTX00840 TGF beta Receptor I antibody. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100
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REFERENCE
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REVIEW
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