Neutralizing:This antibody neutralizes TGF-beta activity in vitro, in an inhibition assay of CCL/64 cell growth and neutralizes the growth promoting action of TGF-beta in the NRK-49F colony forming assay. The effect of microinjection of this antibody into one blastomere of two cell stage Xenopus embryos indicated that it was able to neutralize effectively the bioactivity of TGF-beta in vivo. IHC:This antibody has been used to demonstrate TGF-beta in ovine ovarian tissue and human breast carcinoma at a dilution of 1:1000. As a consequence of the intense staining of the erythrocytes it is possible to locate a single cell within the ovarian stroma making it useful in locating very fine capillary networks within tissue. Western Blotting:This antibody when used at an antibody concentration of 5-20 ng/mL will allow visualization of 100 ng/lane of TGF-beta.
Ovine ovarian tissue, breast cancer sections
Western blotting demonstrated that this antibody reacts with the dimeric (25 Kd) and monomeric (12.5 kDa.) forms of TGF-beta under both non-reducing and reducing conditions respectively.
Preservative: NoneConstituents: 0.01M PBS. pH 7.0
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
1 mg/ml (Please refer to the vial label for the specific concentration.)
TGF-beta 1 from human platelets
Protein G purified.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.