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TGF beta antibody [TB21]

Anti-TGF beta antibody [TB21] used in Western Blot (WB). GTX21279

Cat No. GTX21279

Host

Mouse

Clonality

Monoclonal

Clone Name

TB21

Isotype

IgG1

Application

WB, IHC-P, IHC-Fr, FACS, ELISA, IHC, Neutralizing/Blocking

Reactivity

Human, Mouse, Rat, Rabbit, Sheep, Bovine, Mink, Mustelid
Package
100 μg ($319)

APPLICATION

Application Note

Neutralizing:This antibody neutralizes TGF-beta activity in vitro, in an inhibition assay of CCL/64 cell growth and neutralizes the growth promoting action of TGF-beta in the NRK-49F colony forming assay. The effect of microinjection of this antibody into one blastomere of two cell stage Xenopus embryos indicated that it was able to neutralize effectively the bioactivity of TGF-beta in vivo. IHC:This antibody has been used to demonstrate TGF-beta in ovine ovarian tissue and human breast carcinoma at a dilution of 1:1000. As a consequence of the intense staining of the erythrocytes it is possible to locate a single cell within the ovarian stroma making it useful in locating very fine capillary networks within tissue. Western Blotting:This antibody when used at an antibody concentration of 5-20 ng/mL will allow visualization of 100 ng/lane of TGF-beta.

Positive Control

Ovine ovarian tissue, breast cancer sections

Specificity/Sensitivity

Western blotting demonstrated that this antibody reacts with the dimeric (25 Kd) and monomeric (12.5 kDa.) forms of TGF-beta under both non-reducing and reducing conditions respectively.

PROPERTIES

Form

Liquid

Buffer

Preservative: NoneConstituents: 0.01M PBS. pH 7.0

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

TGF-beta 1 from human platelets

Purification

IgG fraction
Protein G purified.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Background

TGF beta (Transforming Growth Factor beta) is a multifunctional peptide that controls proliferation, differentiation and other functions in many cell types.

Research Area

DATA IMAGES

Anti-TGF beta antibody [TB21] used in Western Blot (WB). GTX21279

GTX21279 WB Image

WB analysis of platelet derived TGF beta at 12mg/lane (lane 2), 6mg/lane (lane 3), 3mg/lane (lane 4) and 1mg/mL (lane 5) with 4-chlorol-napthol as substrate using TGF beta antibody [TB21] at a dilution of 2mg/ml. Lane 1 is molecular weight markers.

REFERENCE

REVIEW

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Application Reference
Clone Reference
Package List Price ($)
$ 319