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TGF beta antibody [TB21]

Orthogonal
Anti-TGF beta antibody [TB21] used in Western Blot (WB). GTX21279
Anti-TGF beta antibody [TB21] used in Western Blot (WB). GTX21279

Cat. No. GTX21279

Reference 21
1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 TB21

Isotype

 IgG1

Application

 WB, IHC-P, IHC-Fr, FACS, ELISA, IHC, Neutralizing/Inhibition

Reactivity

 Human, Mouse, Rat, Rabbit, Sheep, Bovine, Mink, Mustelid
Package
100 μg ($339)
See all TGF beta products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
IHC-P Assay dependent
IHC-Fr Assay dependent
FACS Assay dependent
ELISA Assay dependent
IHC Assay dependent
Neutralizing/Inhibition Assay dependent

Note :

WB
This antibody when used at an antibody concentration of 5-20 ng/mL will allow visualization of 100 ng/lane of TGF-beta.

IHC
This antibody has been used to demonstrate TGF-beta in ovine ovarian tissue and human breast carcinoma at a dilution of 1:1000. As a consequence of the intense staining of the erythrocytes it is possible to locate a single cell within the ovarian stroma making it useful in locating very fine capillary networks within tissue.

Neutralizing/Inhibition
This antibody neutralizes TGF-beta activity in vitro, in an inhibition assay of CCL/64 cell growth and neutralizes the growth promoting action of TGF-beta in the NRK-49F colony forming assay. The effect of microinjection of this antibody into one blastomere of two cell stage Xenopus embryos indicated that it was able to neutralize effectively the bioactivity of TGF-beta in vivo.
Not tested in other applications.

Positive Control

Ovine ovarian tissue, breast cancer sections

Product Note

Western blotting demonstrated that this antibody reacts with the dimeric (25 Kd) and monomeric (12.5 kDa.) forms of TGF-beta under both non-reducing and reducing conditions respectively.

PROPERTIES

Form

Liquid

Buffer

0.01M PBS pH7.0

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

TGF-beta 1 from human platelets

Purification

IgG fraction
Protein G purified.

Conjugation

Unconjugated

RRID

AB_373225

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Background

TGF beta (Transforming Growth Factor beta) is a multifunctional peptide that controls proliferation, differentiation and other functions in many cell types.

Research Area

DATA IMAGES

Anti-TGF beta antibody [TB21] used in Western Blot (WB). GTX21279

GTX21279 WB Image

WB analysis of platelet derived TGF beta at 12mg/lane (lane 2), 6mg/lane (lane 3), 3mg/lane (lane 4) and 1mg/mL (lane 5) with 4-chlorol-napthol as substrate using TGF beta antibody [TB21] at a dilution of 2mg/ml. Lane 1 is molecular weight markers.

Anti-TGF beta antibody [TB21] used in Western Blot (WB). GTX21279

GTX21279 WB Image

Orthogonal

The data was published in the journal J Cell Mol Med in 2012. PMID: 22260151

REFERENCE

Application Reference

Sampaio LP et al. Transl Vis Sci Technol 2023; 12 (9) : 20 Topical Losartan Decreases Myofibroblast Generation But Not Corneal Opacity After Surface Blast-Simulating Irregular PTK in Rabbits.
Hwang JH et al. J Ginseng Res 2023; 47 (5) : 654-661 Skin wound healing effects of (+)-syringaresinol from ginseng berry.
Lee Y et al. Cell Mol Gastroenterol Hepatol 2023; 16 (3) : 411-429 Role of Nox4 in Mitigating Inflammation and Fibrosis in Dextran Sulfate Sodium-Induced Colitis.
Meng N et al. Mol Vis 2023; 29 : 58-67 Basement membrane regeneration and TGF-β1 expression in rabbits with corneal perforating injury.
Sampaio LP et al. Transl Vis Sci Technol 2023; 12 (5) : 15 Cell Biology of Spontaneous Persistent Epithelial Defects After Photorefractive Keratectomy in Rabbits.
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REVIEW

TGF beta antibody [TB21] Cat. No. GTX21279

Rating
( Average 4 based on 1 users reviews)
Application
IHC (Paraffin sections)(IHC-P)
( Average 4 based on 1 users reviews)
Anti-TGF beta antibody [TB21] used in IHC (Paraffin sections) (IHC-P). GTX21279
Date : Anonymous submitted on 05-Oct-2021
Score :
Application Tested : IHC-P
Sample Species : Ms
Sample : mouse skin dermis
Blocking : 1% BSA
Fixation : 4% PFA
Permeabilization /Antigen retrieval : pH9 Nichirei bioscience (lot No. 415211)
Primary Antibody : 1:200, 4°C, > 12Hr
Figure Note : Mouse dermal fibrosis.
Blue : DAPI
Red : TGF beta-1 (Alexa fluor 594). The sample was incubated with 1% BSA containing 1/200 primary antibody for 4ºC overnight. Then, the sample was incubated with 1% BSA containing 1/200 secondary antibody (Alexa fluro 594) for 2 hours at room temperature.
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Application Reference
Sampaio LP et al. Transl Vis Sci Technol 2023; 12 (9):20 Topical Losartan Decreases Myofibroblast Generation But Not Corneal Opacity After Surface Blast-Simulating Irregular PTK in Rabbits.
Hwang JH et al. J Ginseng Res 2023; 47 (5):654-661 Skin wound healing effects of (+)-syringaresinol from ginseng berry.
Lee Y et al. Cell Mol Gastroenterol Hepatol 2023; 16 (3):411-429 Role of Nox4 in Mitigating Inflammation and Fibrosis in Dextran Sulfate Sodium-Induced Colitis.
Meng N et al. Mol Vis 2023; 29:58-67 Basement membrane regeneration and TGF-β1 expression in rabbits with corneal perforating injury.
Na Meng et al. Molecular Vision 2023; 29:58-67 Basement membrane regeneration and TGF-β1 expression in rabbits with corneal perforating injury
Application : IHC-P
Reactivity : Rabbit
Sampaio LP et al. Transl Vis Sci Technol 2023; 12 (5):15 Cell Biology of Spontaneous Persistent Epithelial Defects After Photorefractive Keratectomy in Rabbits.
Sampaio LP et al. Transl Vis Sci Technol 2022; 11 (7):9 Topical Losartan and Corticosteroid Additively Inhibit Corneal Stromal Myofibroblast Generation and Scarring Fibrosis After Alkali Burn Injury.
Naike Casagrande et al. Int J Mol Sci 2021; 22 (6)
Application : FACS
Reactivity : Mouse
de Oliveira RC et al. Exp Eye Res 2021; 202:108325 TGFβ1 and TGFβ2 proteins in corneas with and without stromal fibrosis: Delayed regeneration of apical epithelial growth factor barrier and the epithelial basement membrane in corneas with stromal fibrosis.
Application : IHC-Fr
Reactivity : Rabbit
Licini C et al. Eur J Histochem 2020; 64 (4) Heterotopic ossification in a patient with diffuse idiopathic skeletal hyperostosis: Input from histological findings.
Application : IHC-P
Reactivity : Human
Zhou Y et al. Oncol Lett 2020; 20 (6):298 Anti-CD206 antibody-conjugated Fe3O4-based PLGA nanoparticles selectively promote tumor-associated macrophages to polarize to the pro-inflammatory subtype.
Application : WB
Reactivity : Mouse
Li L et al. Breast Cancer Res 2020; 22 (1):84 Therapeutic role of recurrent ESR1-CCDC170 gene fusions in breast cancer endocrine resistance.
Application : WB
Reactivity : Human
Grabias BM et al. Am J Physiol Renal Physiol 2013; 305 (1):F123-33 Notch4-dependent antagonism of canonical TGF-β1 signaling defines unique temporal fluctuations of SMAD3 activity in sheared proximal tubular epithelial cells.
Application : WB
Reactivity : Human
Lee E et al. Cancer 2013; 119 (9):1752-60 Cohypermethylation of p14 in combination with CADM1 or DCC as a recurrence-related prognostic indicator in stage I esophageal squamous cell carcinoma.
Application : IHC-P
Reactivity : Human
Insalaco L et al. J Cell Mol Med 2012; 16 (9):2186-95 analysis of molecular mechanisms and anti-tumoural effects of * acid in breast cancer cells.
Application : WB
Reactivity : Human
Jung Y et al. Int J Oncol 2012; 40 (4):1122-32 Identification of prognostic biomarkers for glioblastomas using protein expression profiling.
Cayli S et al. Acta Histochem 2012; 114 (1):43441 Jab1 expression is associated with TGF-β1 signaling in chronic rhinosinusitis and nasal polyposis.
Application : IHC
Reactivity : Human
Hakim SG et al. Radiother Oncol 2011; 101 (1):34213 Expression of Wnt-1, TGF-β and related cell-cell adhesion components following radiotherapy in salivary glands of patients with manifested radiogenic xerostomia.
Crawford SE et al. Cell 1998; 93 (7):1159-70 Thrombospondin-1 is a major activator of TGF-beta1 in vivo.
Clone Reference
Carlin GL et al. Eur J Obstet Gynecol Reprod Biol X 2020; 7:100111 The role of transforming growth factor-? (TGF-?1) in postmenopausal women with pelvic organ prolapse: An immunohistochemical study.
Hu HY et al. Int J Clin Exp Pathol 2016; 9 (6):6171-6178 ATRA alleviated endometrial fibrosis in a rabbit intrauterine adhesions model through downregulation of the TGF-β1/smad4 signaling pathway.
Application : WB, IHC-P
Reactivity : Rabbit
SDS
PBS.pdf
Package List Price ($)
$ 339
Custom Conjugations