GeneTex
United States (US)

TGFBI antibody [C1C3]

GTX100744_WB_19011506_480.jpg

Cat No. GTX100744

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human, Chicken
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

75 kDa. ( Note )

Positive Control

HeLa

Predict Reactivity

Mouse, Rat, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TGFBI. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Transforming Growth Factor Beta Induced,Bigh3,Cdb1,Cdg2,Cdgg1,Csd,Csd1,Csd2,Csd3,Ebmd,Lcd1,Tgfbi

Cellular Localization

Secreted , extracellular space , extracellular matrix

Background

This gene encodes an RGD-containing protein that binds to type I, II and IV collagens. The RGD motif is found in many extracellular matrix proteins modulating cell adhesion and serves as a ligand recognition sequence for several integrins. This protein plays a role in cell-collagen interactions and may be involved in endochondrial bone formation in cartilage. The protein is induced by transforming growth factor-beta and acts to inhibit cell adhesion. Mutations in this gene are associated with multiple types of corneal dystrophy. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX100744_WB_19011506_480.jpg

GTX100744 WB Image

Sample (30 µg of whole cell lysate)
A: HeLa
7.5% SDS PAGE
TGFBI antibody
GTX100744 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
REVIEW

TGFBI antibody [C1C3] Cat No. GTX100744

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 24-May-2018
Score :
Application Tested : WB
Sample Species : Hu
Sample : OC3 cells
Amount used : 100 μg
Blocking : 5 % milk, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169