GeneTex
United States (US)

TGFBI antibody, Internal

GTX88213_2465_WB_18121411_893.jpg
GTX88213_1030_IHC-P_18121411_350.jpg

Cat No. GTX88213

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.2-0.5μg/ml
IHC-P 4-6μg/ml

Note :

IHC-P
Kidney shows staining of intercalated cells of a collecting tubule.

Not tested in other applications.

Calculated MW

75 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88213-PEP).

Predict Reactivity

Mouse, Rat, Dog(>80% identity)
PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-QLYTDRTEKLRPE, from the internal region of the protein sequence according to NP_000349.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Transforming Growth Factor Beta Induced,Bigh3,Cdb1,Cdg2,Cdgg1,Csd,Csd1,Csd2,Csd3,Ebmd,Lcd1,Tgfbi

Cellular Localization

May be associated both with microfibrils and with the cell surface.

Background

This gene encodes an RGD-containing protein that binds to type I, II and IV collagens. The RGD motif is found in many extracellular matrix proteins modulating cell adhesion and serves as a ligand recognition sequence for several integrins. This protein plays a role in cell-collagen interactions and may be involved in endochondrial bone formation in cartilage. The protein is induced by transforming growth factor-beta and acts to inhibit cell adhesion. Mutations in this gene are associated with multiple types of corneal dystrophy. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
GTX88213_2465_WB_18121411_893.jpg

GTX88213 WB Image

WB analysis of human kidney lysate using GTX88213 TGFBI antibody, Internal.
Dilution : 0.3µg/ml
Loading : 35µg protein in RIPA buffer

GTX88213_1030_IHC-P_18121411_350.jpg

GTX88213 IHC-P Image

IHC-P analysis of human kidney using GTX88213 TGFBI antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.8µg/ml

Package List Price ($)
$ 329