GeneTex
United States (US)

TGR5 antibody [C2C3], C-term

GTX100026_WB_19011505_973.jpg
GTX100026_39233_IHC_19011506_818.jpg

Cat No. GTX100026

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

35 kDa. ( Note )

Positive Control

A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji

Predict Reactivity

Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human TGR5. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

BG37 antibody, GPCR19 antibody, GPR131 antibody, M-BAR antibody, MGC40597 antibody, TGR5 antibody, GPBAR1 antibody, hBG37 antibody, membrane-type receptor for bile acids antibody, G-protein coupled bile acid receptor 1 antibody, G-protein coupled receptor GPCR19 antibody, hGPCR19 antibody, membrane bile acid receptor antibody, G-protein coupled bile acid receptor BG37 antibody, G protein-coupled bile acid receptor 1 antibody

Cellular Localization

Cell membrane; Multi-pass membrane protein

Background

This gene encodes a member of the G protein-coupled receptor (GPCR) superfamily. This enzyme functions as a cell surface receptor for bile acids. Treatment of cells expressing this GPCR with bile acids induces the production of intracellular cAMP, activation of a MAP kinase signaling pathway, and internalization of the receptor. The receptor is implicated in the suppression of macrophage functions and regulation of energy homeostasis by bile acids. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq]

Database

DATA IMAGES
GTX100026_WB_19011505_973.jpg

GTX100026 WB Image

Sample (30 µg of whole cell lysate)
A: HepG2 (GTX27900)
10% SDS PAGE
GTX100026 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100026_39233_IHC_19011506_818.jpg

GTX100026 IHC-P Image

Immunohistochemical analysis of paraffin-embedded HUH-7 xenograft, using TGR5(GTX100026) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Document
Unmodified.pdf
Package List Price ($)
$ 319
$ 169