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TGR5 antibody [C2C3], C-term

Anti-TGR5 antibody [C2C3], C-term used in Western Blot (WB). GTX100026
Anti-TGR5 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100026
Anti-TGR5 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100026

Cat. No. GTX100026

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all TGR5 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

35 kDa. ( Note )

Positive Control

HepG2

Predict Reactivity

Rabbit, Bovine, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide encompassing a sequence within the Intracellular domain of human TGR5. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950435

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

G protein-coupled bile acid receptor 1 , BG37 , GPCR19 , GPR131 , M-BAR , TGR5

Cellular Localization

Cell membrane; Multi-pass membrane protein

Background

This gene encodes a member of the G protein-coupled receptor (GPCR) superfamily. This enzyme functions as a cell surface receptor for bile acids. Treatment of cells expressing this GPCR with bile acids induces the production of intracellular cAMP, activation of a MAP kinase signaling pathway, and internalization of the receptor. The receptor is implicated in the suppression of macrophage functions and regulation of energy homeostasis by bile acids. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-TGR5 antibody [C2C3], C-term used in Western Blot (WB). GTX100026

GTX100026 WB Image

Sample (30 μg of whole cell lysate)
A: HepG2 (GTX27900)
10% SDS PAGE
GTX100026 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TGR5 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100026

GTX100026 IHC-P Image

Immunohistochemical analysis of paraffin-embedded HUH-7 xenograft, using TGR5(GTX100026) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-TGR5 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100026

GTX100026 IHC-P Image

The data was published in the journal Cancers (Basel) in 2019.PMID: 31461945

REFERENCE

Application Reference

P Kov?cs et al. Cancers (Basel) 2019; 11 (9) : 1255 Lithocholic Acid, a Metabolite of the Microbiome, Increases Oxidative Stress in Breast Cancer
Application : IHC-P
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Document
Unmodified.pdf
Package List Price ($)
$ 399
$ 169