APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Dilution |
1 - 2 μg/mL |
2.5 μg/mL |
Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
Human Liver Tissue Slide , Human Liver Tissue Lysate
Product Note
The additional higher molecular weight bands seen in the immunoblot may represent post-translationally modified TMEM4.
PROPERTIES
Form
Liquid
Buffer
PBS with 0.02% sodium azide.
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
THEM4 antibody was raised against an 18 amino acid synthetic peptide near the center of human THEM4.The immunogen is located within amino acids 60 - 110 of THEM4.
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
thioesterase superfamily member 4 , CTMP
Cellular Localization
Cell membrane,Cytoplasm,Mitochondrion
Background
Protein kinase B (PKB) is a major downstream target of receptor tyrosine kinases that signal via phosphatidylinositol 3-kinase. Upon cell stimulation, PKB is translocated to the plasma membrane, where it is phosphorylated in the C-terminal regulatory domain. The protein encoded by this gene negatively regulates PKB activity by inhibiting phosphorylation. Transcription of this gene is commonly downregulated in glioblastomas. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX31383 WB Image
WB analysis of human liver tissue lysate using GTX31383 THEM4 antibody. Working concentration : (A) 1 and (B) 2 μg/ml
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GTX31383 IHC-P Image
IHC-P analysis of human liver tissue using GTX31383 THEM4 antibody. Working concentration : 2.5 μg/ml
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GTX31383 IHC-P Image
IHC-P analysis of human liver tissue using GTX31383 THEM4 antibody. Working concentration : 20 μg/ml
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REFERENCE
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REVIEW
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