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THEM4 antibody

Anti-THEM4 antibody used in Western Blot (WB). GTX31383
Anti-THEM4 antibody used in IHC (Paraffin sections) (IHC-P). GTX31383
Anti-THEM4 antibody used in IHC (Paraffin sections) (IHC-P). GTX31383

Cat No. GTX31383

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P, ELISA

Reactivity

Human, Mouse, Rat
Package
100 μg ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1 - 2 μg/mL
IHC-P 2.5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

27 kDa. ( Note )

Positive Control

Human Liver Tissue Slide , Human Liver Tissue Lysate

Specificity/Sensitivity

The additional higher molecular weight bands seen in the immunoblot may represent post-translationally modified TMEM4.

PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

THEM4 antibody was raised against an 18 amino acid synthetic peptide near the center of human THEM4.The immunogen is located within amino acids 60 - 110 of THEM4.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

thioesterase superfamily member 4 , CTMP

Cellular Localization

Cell membrane,Cytoplasm,Mitochondrion

Background

Protein kinase B (PKB) is a major downstream target of receptor tyrosine kinases that signal via phosphatidylinositol 3-kinase. Upon cell stimulation, PKB is translocated to the plasma membrane, where it is phosphorylated in the C-terminal regulatory domain. The protein encoded by this gene negatively regulates PKB activity by inhibiting phosphorylation. Transcription of this gene is commonly downregulated in glioblastomas. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-THEM4 antibody used in Western Blot (WB). GTX31383

GTX31383 WB Image

WB analysis of human liver tissue lysate using GTX31383 THEM4 antibody.
Working concentration : (A) 1 and (B) 2 μg/ml

Anti-THEM4 antibody used in IHC (Paraffin sections) (IHC-P). GTX31383

GTX31383 IHC-P Image

IHC-P analysis of human liver tissue using GTX31383 THEM4 antibody.
Working concentration : 2.5 μg/ml

Anti-THEM4 antibody used in IHC (Paraffin sections) (IHC-P). GTX31383

GTX31383 IHC-P Image

IHC-P analysis of human liver tissue using GTX31383 THEM4 antibody.
Working concentration : 20 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 339