TIA1 antibody, Internal

Cat. No. GTX89059

Host	Goat
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human
Package	100 μg ($399)

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Datasheet
Document

View product citations for antibody GTX89059 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	0.1-0.3μg/ml
ICC/IF	5μg/ml
IHC-P	3.75μg/ml
Note : ICC/IF Strong expression of the protein seen in the nucleus and cytoplasm of HeLa cells.

Not tested in other applications.

Calculated MW

43 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX89059-PEP).

Product Note

This antibody is expected to recognise both reported isoforms (NP_071320.1 and NP_071505.1).

Predict Reactivity

Mouse, Rat, Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-PKSTYESNTKQ, from the internal region of the protein sequence according to NP_071320.1; NP_071505.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

TIA1 cytotoxic granule associated RNA binding protein , TIA-1 , WDM

Cellular Localization

Nucleus

Background

The product encoded by this gene is a member of a RNA-binding protein family and possesses nucleolytic activity against cytotoxic lymphocyte (CTL) target cells. It has been suggested that this protein may be involved in the induction of apoptosis as it preferentially recognizes poly(A) homopolymers and induces DNA fragmentation in CTL targets. The major granule-associated species is a 15-kDa protein that is thought to be derived from the carboxyl terminus of the 40-kDa product by proteolytic processing. Alternative splicing resulting in different isoforms has been found for this gene. [provided by RefSeq, May 2017]

Database

Gene ID: 7072 TIA1
UniProt: P31483 TIA1

Research Area

DATA IMAGES

GTX89059 ICC/IF Image

ICC/IF analysis of PFA fixed HeLa cells using GTX89059 TIA1 antibody, Internal.
Green : Primary antibody
Blue : DAPI
Negative control : Unimmunized goat IgG
Permeabilization : 0.15% Triton
Dilution : 5μg/ml

GTX89059 IHC-P Image

IHC-P analysis of human thyroid using GTX89059 TIA1 antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.75μg/ml

GTX89059 WB Image

WB analysis of Jurkat cell lysate using GTX89059 TIA1 antibody, Internal.
Dilution : 0.1μg/ml
Loading : 35μg protein in RIPA buffer

REFERENCE

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SDS
Sodium Azide.pdf

Package

List Price ($)

100 μg

$ 399

Qty