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TIA1 antibody [N1N3]

Anti-TIA1 antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113280
Anti-TIA1 antibody [N1N3] used in Western Blot (WB). GTX113280

Cat No. GTX113280

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

43 kDa. ( Note )

Positive Control

A549 , 293T , A431 , HeLa , HepG2 , Molt-4

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine, Chicken, Xenopus laevis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TIA1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952245

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

TIA1 cytotoxic granule associated RNA binding protein , TIA-1 , WDM

Cellular Localization

Cytoplasmic granule

Background

The product encoded by this gene is a member of a RNA-binding protein family and possesses nucleolytic activity against cytotoxic lymphocyte (CTL) target cells. It has been suggested that this protein may be involved in the induction of apoptosis as it preferentially recognizes poly(A) homopolymers and induces DNA fragmentation in CTL targets. The major granule-associated species is a 15-kDa protein that is thought to be derived from the carboxyl terminus of the 40-kDa product by proteolytic processing. Alternative splicing resulting in different isoforms of this gene product has been described in the literature. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-TIA1 antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113280

GTX113280 ICC/IF Image

TIA1 antibody [N1N3] detects TIA1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TIA1 stained by TIA1 antibody [N1N3] (GTX113280) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:100.
Scale bar= 10μm.

Anti-TIA1 antibody [N1N3] used in Western Blot (WB). GTX113280

GTX113280 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
12% SDS PAGE
GTX113280 diluted at 1:1000

REFERENCE

There are currently no references for TIA1 antibody [N1N3] (GTX113280). Be the first to share your publications with this product.

REVIEW

TIA1 antibody [N1N3] Cat No. GTX113280

Rating ( Average 4 based on 1 users reviews)
Application
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 04-Nov-2011
Score :
Application Tested : ICC/IF
Sample Species : Hu
Sample : A549 cells
Blocking : 1% BSA in PBST, 25°C, 0.5Hr
Fixation : 4%PFA
Permeabilization /Antigen retrieval : 0.25% Triton X-100
Primary Antibody : 1:100, 25°C, 4Hr
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169