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TID1 antibody [N3C3]

Anti-TID1 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX111077
Anti-TID1 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX111077
Anti-TID1 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111077
Anti-TID1 antibody [N3C3] used in Western Blot (WB). GTX111077

Cat. No. GTX111077

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($419),
25 μl ($169)
See all TID1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji

Predict Reactivity

Bovine, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TID1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950125

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

DnaJ heat shock protein family (Hsp40) member A3 , HCA57 , TID1 , hTID-1

Cellular Localization

Mitochondrion matrix

Background

DNAJA3 belongs to the evolutionarily conserved DNAJ/HSP40 family of proteins. For background information on the DNAJ family, see MIM 608375.[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-TID1 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX111077

GTX111077 IHC-P Image

Immunohistochemical analysis of paraffin-embedded MCF7 xenograft, using TID1(GTX111077) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-TID1 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX111077

GTX111077 IHC-P Image

TID1 antibody [N3C3] detects TID1 protein at cytoplasm in rat muscle by immunohistochemical analysis.
Sample: Paraffin-embedded rat muscle.
TID1 antibody [N3C3] (GTX111077) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-TID1 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111077

GTX111077 ICC/IF Image

Immunofluorescence analysis of methanol-fixed MCF-7, using TID1(GTX111077) antibody at 1:500 dilution.

Anti-TID1 antibody [N3C3] used in Western Blot (WB). GTX111077

GTX111077 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
B: A431 (GTX27909)
C: H1299
D: Hela
E: Hep G2 (GTX27900)
F: Molt-4 (GTX27912)
G: Raji
10% SDS PAGE
GTX111077 diluted at 1:1000

REFERENCE

Application Reference

Salazar-Petres E et al. Biol Reprod 2022; 106 (6) : 1292-1311 Placental structure, function, and mitochondrial phenotype relate to fetal size in each fetal sex in mice?.
Application : WB
Reactivity : Mouse
Nuzzo AM et al. Am J Pathol 2018; (Epub) Placental Adaptation to Early-Onset Hypoxic Pregnancy and Mitochondria-Targeted Antioxidant Therapy in Rat.
Application : IHC-P
Reactivity : Rat
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 419
$ 169