GeneTex
United States (US)

TID1 antibody

GTX100608_40639_IFA_19011506_695.jpg
GTX100608_40639_WB_19011506_802.jpg
GTX100608_40639_IHC_19011506_699.jpg

Cat No. GTX100608

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

Positive Control

HepG2 , HCT116

Predict Reactivity

Mouse, Rat, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.46mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TID1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

FLJ45758 antibody, TID1 antibody, hTid-1 antibody, DNAJA3 antibody, tumorous imaginal discs protein Tid56 homolog antibody, hepatocellular carcinoma-associated antigen 57 antibody, "dnaJ homolog subfamily A member 3, mitochondrial antibody", dnaJ protein Tid-1 antibody, "DnaJ (Hsp40) homolog, subfamily A, member 3 antibody"

Cellular Localization

Mitochondrion matrix

Background

DNAJA3 belongs to the evolutionarily conserved DNAJ/HSP40 family of proteins. For background information on the DNAJ family, see MIM 608375.[supplied by OMIM]

Database

Research Area

DATA IMAGES
GTX100608_40639_IFA_19011506_695.jpg

GTX100608 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using TID1(GTX100608) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

GTX100608_40639_WB_19011506_802.jpg

GTX100608 WB Image

Sample (30 ug of whole cell lysate)
A: HepG2
B: HCT116
10% SDS PAGE
GTX100608 diluted at 1:1000

GTX100608_40639_IHC_19011506_699.jpg

GTX100608 IHC-P Image

Immunohistochemical analysis of paraffin-embedded BT483 xenograft, using TID1(GTX100608) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169