GeneTex
United States (US)

TIE1 antibody

GTX103429_41766_20150827_WB_19011506_197.jpg
GTX103429_40401_IHC_19011506_566.jpg

Cat No. GTX103429

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

125 kDa. ( Note )

Positive Control

HUVEC

Predict Reactivity

Mouse, Rat, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TIE1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

JTK14 antibody, TIE antibody, TIE1 antibody, tyrosine kinase with immunoglobulin and epidermal growth factor homology domains 1 antibody, tyrosine-protein kinase receptor Tie-1 antibody, tyrosine kinase with immunoglobulin-like and EGF-like domains 1 antibody

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

Probable protein tyrosine-kinase transmembrane receptor.

Database

Research Area

DATA IMAGES
GTX103429_41766_20150827_WB_19011506_197.jpg

GTX103429 WB Image

TIE1 antibody detects TIE1 protein by western blot analysis. Whole cell extracts (30 µg) was separated by 5% SDS-PAGE, and the membrane was blotted with TIE1 antibody (GTX103429) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX103429_40401_IHC_19011506_566.jpg

GTX103429 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using TIE1(GTX103429) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169