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TIE1 antibody

Anti-TIE1 antibody used in IHC (Paraffin sections) (IHC-P). GTX103429
Anti-TIE1 antibody used in Western Blot (WB). GTX103429

Cat. No. GTX103429

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

125 kDa. ( Note )

Positive Control

HUVEC

Predict Reactivity

Mouse, Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TIE1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10729206

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

tyrosine kinase with immunoglobulin like and EGF like domains 1 , JTK14 , TIE

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

Probable protein tyrosine-kinase transmembrane receptor.

Database

Research Area

DATA IMAGES

Anti-TIE1 antibody used in IHC (Paraffin sections) (IHC-P). GTX103429

GTX103429 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using TIE1(GTX103429) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-TIE1 antibody used in Western Blot (WB). GTX103429

GTX103429 WB Image

TIE1 antibody detects TIE1 protein by western blot analysis. Whole cell extracts (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with TIE1 antibody (GTX103429) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169