GeneTex
United States (US)

TIE1 antibody

Overexpression
GTX107818_40660_WB_19011507_903.jpg
GTX107818_40660_WB_B_19011507_292.jpg
GTX107818_40660_IHC_19011507_343.jpg

Cat No. GTX107818

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

125 kDa. ( Note )

Positive Control

Jurkat , Raji , K562 , human TIE1-transfected 293T cells

Predict Reactivity

Rat, Zebrafish, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human TIE1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

JTK14 antibody, TIE antibody, TIE1 antibody, tyrosine kinase with immunoglobulin and epidermal growth factor homology domains 1 antibody, tyrosine-protein kinase receptor Tie-1 antibody, tyrosine kinase with immunoglobulin-like and EGF-like domains 1 antibody

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

Probable protein tyrosine-kinase transmembrane receptor.
Show More +

Database

Research Area

DATA IMAGES
GTX107818_40660_WB_19011507_903.jpg

GTX107818 WB Image

TIE1 antibody detects TIE1 protein by Western blot analysis.
A. 30 µg Jurkat whole cell lysate/extract
B. 30 µg Raji whole cell lysate/extract
C. 30 µg K562 whole cell lysate/extract
5 % SDS-PAGE
TIE1 antibody (GTX107818) dilution: 1:1000

GTX107818_40660_WB_B_19011507_292.jpg

GTX107818 WB Image

Overexpression

TIE1 antibody detects TIE1 protein by Western blot analysis.
A. 30 µg 293T whole cell lysate/extract
B. 30 µg whole cell lysate/extract of human TIE1-transfected 293T cells
5 % SDS-PAGE
TIE1 antibody (GTX107818) dilution: 1:5000

GTX107818_40660_IHC_19011507_343.jpg

GTX107818 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using TIE1(GTX107818) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Reference
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf