GeneTex
United States (US)

TIE1 antibody

Anti-TIE1 antibody used in Western Blot (WB). GTX107818
Anti-TIE1 antibody used in Western Blot (WB). GTX107818
Anti-TIE1 antibody used in IHC (Paraffin sections) (IHC-P). GTX107818

Cat No. GTX107818

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

125 kDa. ( Note )

Positive Control

Jurkat , Raji , K562 , human TIE1-transfected 293T cells

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human TIE1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Tyrosine Kinase With Immunoglobulin Like And Egf Like Domains 1,Jtk14,Tie,Tie1

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

Probable protein tyrosine-kinase transmembrane receptor.

Database

Research Area

DATA IMAGES
Anti-TIE1 antibody used in Western Blot (WB). GTX107818

GTX107818 WB Image

TIE1 antibody detects TIE1 protein by Western blot analysis.
A. 30 µg Jurkat whole cell lysate/extract
B. 30 µg Raji whole cell lysate/extract
C. 30 µg K562 whole cell lysate/extract
5 % SDS-PAGE
TIE1 antibody (GTX107818) dilution: 1:1000

Anti-TIE1 antibody used in Western Blot (WB). GTX107818

GTX107818 WB Image

TIE1 antibody detects TIE1 protein by Western blot analysis.
A. 30 µg 293T whole cell lysate/extract
B. 30 µg whole cell lysate/extract of human TIE1-transfected 293T cells
5 % SDS-PAGE
TIE1 antibody (GTX107818) dilution: 1:5000

Anti-TIE1 antibody used in IHC (Paraffin sections) (IHC-P). GTX107818

GTX107818 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using TIE1(GTX107818) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169