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TIE1 antibody

Orthogonal Overexpression
Anti-TIE1 antibody used in Western Blot (WB). GTX107818
Anti-TIE1 antibody used in Western Blot (WB). GTX107818
Anti-TIE1 antibody used in IHC (Paraffin sections) (IHC-P). GTX107818
Anti-TIE1 antibody used in Western Blot (WB). GTX107818

Cat. No. GTX107818

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all TIE1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

125 kDa. ( Note )

Positive Control

human TIE1-transfected 293T cells , Jurkat , Raji , K562

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the Intracellular domain of human TIE1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2038122

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

tyrosine kinase with immunoglobulin like and EGF like domains 1 , JTK14 , TIE

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

Probable protein tyrosine-kinase transmembrane receptor.

Database

Research Area

DATA IMAGES

Anti-TIE1 antibody used in Western Blot (WB). GTX107818

GTX107818 WB Image

Overexpression

TIE1 antibody detects TIE1 protein by Western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg whole cell lysate/extract of human TIE1-transfected 293T cells
5 % SDS-PAGE
TIE1 antibody (GTX107818) dilution: 1:5000

Anti-TIE1 antibody used in Western Blot (WB). GTX107818

GTX107818 WB Image

TIE1 antibody detects TIE1 protein by Western blot analysis.
A. 30 μg Jurkat whole cell lysate/extract
B. 30 μg Raji whole cell lysate/extract
C. 30 μg K562 whole cell lysate/extract
5 % SDS-PAGE
TIE1 antibody (GTX107818) dilution: 1:1000

Anti-TIE1 antibody used in IHC (Paraffin sections) (IHC-P). GTX107818

GTX107818 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using TIE1(GTX107818) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-TIE1 antibody used in Western Blot (WB). GTX107818

GTX107818 WB Image

Orthogonal

The data was published in the Oncotarget in 2018. PMID: 29435158

REFERENCE

Application Reference

Fan CS et al. Oncotarget 2018; 9 (4) : 4998-5015 Osteopontin-integrin engagement induces HIF-1α-TCF12-mediated endothelial-mesenchymal transition to exacerbate colorectal cancer.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169