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TIE2 antibody

Orthogonal
Anti-TIE2 antibody used in Western Blot (WB). GTX107505
Anti-TIE2 antibody used in Western Blot (WB). GTX107505

Cat. No. GTX107505

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all TIE2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

126 kDa. ( Note )

Predict Reactivity

Mouse, Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.49 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the Extracellular domain of human TIE2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036496

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

TEK receptor tyrosine kinase , CD202B , GLC3E , TIE-2 , TIE2 , VMCM , VMCM1

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

The TEK receptor tyrosine kinase is expressed almost exclusively in endothelial cells in mice, rats, and humans. This receptor possesses a unique extracellular domain containing 2 immunoglobulin-like loops separated by 3 epidermal growth factor-like repeats that are connected to 3 fibronectin type III-like repeats. The ligand for the receptor is angiopoietin-1. Defects in TEK are associated with inherited venous malformations; the TEK signaling pathway appears to be critical for endothelial cell-smooth muscle cell communication in venous morphogenesis. TEK is closely related to the TIE receptor tyrosine kinase. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-TIE2 antibody used in Western Blot (WB). GTX107505

GTX107505 WB Image

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with TIE2 antibody (GTX107505) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TIE2 antibody used in Western Blot (WB). GTX107505

GTX107505 WB Image

Orthogonal

The data was published in the Oncotarget in 2018. PMID: 29435158

REFERENCE

Application Reference

Mirando AC et al. JCI Insight 2019; (Epub) A collagen IV-derived peptide disrupts α5β1 integrin and potentiates Ang2-Tie2 signaling.
Fan CS et al. Oncotarget 2018; 9 (4) : 4998-5015 Osteopontin-integrin engagement induces HIF-1α-TCF12-mediated endothelial-mesenchymal transition to exacerbate colorectal cancer.
Application : WB
Reactivity : Human
Chuan-Chen Ho et al. J. Mater. Chem. B 2015; In vitro degradation and angiogenesis of porous calcium silicate-gelatin composite scaffold.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169