GeneTex
United States (US)

TIE2 antibody

GTX64389_20180530_ICCIF_18121411_237.jpg
GTX64389_20180530_WB_18121411_345.jpg

Cat No. GTX64389

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:100
Not tested in other applications.

Calculated MW

126 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant funsion protein containing a sequence corresponding to amino acids 500-750 of human TEK (NP_000450.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

VMCM Antibody , TEK Antibody , VMCM1 Antibody , CD202B Antibody

Background

The TEK receptor tyrosine kinase is expressed almost exclusively in endothelial cells in mice, rats, and humans. This receptor possesses a unique extracellular domain containing 2 immunoglobulin-like loops separated by 3 epidermal growth factor-like repeats that are connected to 3 fibronectin type III-like repeats. The ligand for the receptor is angiopoietin-1. Defects in TEK are associated with inherited venous malformations; the TEK signaling pathway appears to be critical for endothelial cell-smooth muscle cell communication in venous morphogenesis. TEK is closely related to the TIE receptor tyrosine kinase. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
GTX64389_20180530_ICCIF_18121411_237.jpg

GTX64389 ICC/IF Image

ICC/IF analysis of MCF-7 cell using GTX64389 TIE2 antibody.
Blue : DAPI

GTX64389_20180530_WB_18121411_345.jpg

GTX64389 WB Image

WB analysis of various samples using GTX64389 TIE2 antibody.
Dilution : 1:1000
Loading : 25μg per lane