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TIE2 (phospho Ser1119) antibody

Anti-TIE2 (phospho Ser1119) antibody used in Western Blot (WB). GTX00793
Anti-TIE2 (phospho Ser1119) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00793
Anti-TIE2 (phospho Ser1119) antibody used in ELISA (ELISA). GTX00793

Cat No. GTX00793

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, ELISA

Reactivity

Human, Mouse
Package
100 μl ($329)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:3000
ICC/IF 1:100-1:500
ELISA Assay dependent
Not tested in other applications.

Calculated MW

126 kDa. ( Note )

Predict Reactivity

Zebrafish, Rabbit, Sheep, Bovine, Chicken, Pig, Horse, Xenopus(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS pH 7.4, 150mM NaCl, 0.02% sodium azide, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human TIE2 around the phosphorylation site of Ser1119.

Purification

Purified by sequential chromatography on phospho-peptide and non-phospho-peptide columns
From serum

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

CD202B,TEK,TEK receptor tyrosine kinase,TIE2,VMCM,VMCM1

Cellular Localization

Cell membrane,Cell junction,Focal adhesion,Cytoplasm,Cytoskeleton,Secreted

Background

This gene encodes a receptor that belongs to the protein tyrosine kinase Tie2 family. The encoded protein possesses a unique extracellular region that contains two immunoglobulin-like domains, three epidermal growth factor (EGF)-like domains and three fibronectin type III repeats. The ligand angiopoietin-1 binds to this receptor and mediates a signaling pathway that functions in embryonic vascular development. Mutations in this gene are associated with inherited venous malformations of the skin and mucous membranes. Alternative splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Feb 2014]

Database

Research Area

DATA IMAGES

Anti-TIE2 (phospho Ser1119) antibody used in Western Blot (WB). GTX00793

GTX00793 WB Image

WB analysis of EGF treated 293 cell lysates using GTX00793 TIE2 (Phospho-Ser1119) antibody pre-incubated with phospho- or non-phospho- peptide.
P-peptide : phospho-peptide
N-peptide : non-phospho-peptide

Anti-TIE2 (phospho Ser1119) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00793

GTX00793 ICC/IF Image

ICC/IF analysis of PFA-fixed NIH-3T3 cells using GTX00793 TIE2 (Phospho-Ser1119) antibody.
Red : Primary antibody
Permeabilization : 0.1% Triton X-100
Dilution : 1:200

Anti-TIE2 (phospho Ser1119) antibody used in ELISA (ELISA). GTX00793

GTX00793 ELISA Image

ELISA analysis of phospho- and non phosho- immunogen peptide using GTX00793 Phospho-TIE2 (Ser1119) antibody.
P-peptide : phospho-peptide
N-peptide : non-phospho-peptide
Peptides concentration : 1μg/ml

REFERENCE

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REVIEW

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Package List Price ($)
$ 329