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TIE2 (phospho Tyr897) antibody

Anti-TIE2 (phospho Tyr897) antibody used in IHC (Paraffin sections) (IHC-P). GTX00795
Anti-TIE2 (phospho Tyr897) antibody used in ELISA (ELISA). GTX00795
Anti-TIE2 (phospho Tyr897) antibody used in IHC (Paraffin sections) (IHC-P). GTX00795
Anti-TIE2 (phospho Tyr897) antibody used in Western Blot (WB). GTX00795

Cat No. GTX00795

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P, ELISA

Reactivity

Human, Mouse
Package
100 μl ($329)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:2000
IHC-P 1:50-1:200
ELISA Assay dependent
Not tested in other applications.

Calculated MW

126 kDa. ( Note )

Predict Reactivity

Zebrafish, Rabbit, Sheep, Bovine, Chicken, Pig, Horse, Xenopus(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS pH 7.4, 150mM NaCl, 0.02% sodium azide, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human Tie2 around the phosphorylation site of Tyr897.

Purification

Purified by sequential chromatography on phospho-peptide and non-phospho-peptide columns
From serum

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

CD202B,TEK,TEK receptor tyrosine kinase,TIE2,VMCM,VMCM1

Cellular Localization

Cell membrane,Cell junction,Focal adhesion,Cytoplasm,Cytoskeleton,Secreted

Background

This gene encodes a receptor that belongs to the protein tyrosine kinase Tie2 family. The encoded protein possesses a unique extracellular region that contains two immunoglobulin-like domains, three epidermal growth factor (EGF)-like domains and three fibronectin type III repeats. The ligand angiopoietin-1 binds to this receptor and mediates a signaling pathway that functions in embryonic vascular development. Mutations in this gene are associated with inherited venous malformations of the skin and mucous membranes. Alternative splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Feb 2014]

Database

Research Area

DATA IMAGES

Anti-TIE2 (phospho Tyr897) antibody used in IHC (Paraffin sections) (IHC-P). GTX00795

GTX00795 IHC-P Image

IHC-P analysis of mouse heart tissue using GTX00795 TIE2 (phospho Tyr897) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution : 1:100

Anti-TIE2 (phospho Tyr897) antibody used in ELISA (ELISA). GTX00795

GTX00795 ELISA Image

ELISA analysis of phospho- and non phosho- immunogen peptide using GTX00795 TIE2 (phospho Tyr897) antibody.
P-peptide : phospho-peptide
N-peptide : non-phospho-peptide
Peptides concentration : 1μg/ml

Anti-TIE2 (phospho Tyr897) antibody used in IHC (Paraffin sections) (IHC-P). GTX00795

GTX00795 IHC-P Image

IHC-P analysis of human liver tissue using GTX00795 TIE2 (phospho Tyr897) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution : 1:100

Anti-TIE2 (phospho Tyr897) antibody used in Western Blot (WB). GTX00795

GTX00795 WB Image

WB analysis of NIH-3T3 cell lysates using GTX00795 TIE2 (phospho Tyr897) antibody pre-incubated with phospho- or non-phospho- peptide.
P-peptide : phospho-peptide
N-peptide : non-phospho-peptide

REFERENCE

There are currently no references for TIE2 (phospho Tyr897) antibody (GTX00795). Be the first to share your publications with this product.

REVIEW

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Package List Price ($)
$ 329