APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:2000 |
| 1:50-1:200 |
| Assay dependent |
Not tested in other applications.
Calculated MW
Predict Reactivity
Zebrafish, Rabbit, Sheep, Bovine, Chicken, Pig, Horse, Xenopus(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 150mM NaCl, 50% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
A synthesized peptide derived from human Tie2 around the phosphorylation site of Tyr897.
Purification
Purified by sequential chromatography on phospho-peptide and non-phospho-peptide columns
From serum
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
CD202B , TEK , TEK receptor tyrosine kinase , TIE2 , VMCM , VMCM1
Cellular Localization
Cell membrane,Cell junction,Focal adhesion,Cytoplasm,Cytoskeleton,Secreted
Background
This gene encodes a receptor that belongs to the protein tyrosine kinase Tie2 family. The encoded protein possesses a unique extracellular region that contains two immunoglobulin-like domains, three epidermal growth factor (EGF)-like domains and three fibronectin type III repeats. The ligand angiopoietin-1 binds to this receptor and mediates a signaling pathway that functions in embryonic vascular development. Mutations in this gene are associated with inherited venous malformations of the skin and mucous membranes. Alternative splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Feb 2014]
Database
Research Area
DATA IMAGES
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GTX00795 IHC-P Image
IHC-P analysis of mouse heart tissue using GTX00795 TIE2 (phospho Tyr897) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution : 1:100
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GTX00795 ELISA Image
ELISA analysis of phospho- and non phosho- immunogen peptide using GTX00795 TIE2 (phospho Tyr897) antibody.
P-peptide : phospho-peptide
N-peptide : non-phospho-peptide
Peptides concentration : 1μg/ml
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GTX00795 IHC-P Image
IHC-P analysis of human liver tissue using GTX00795 TIE2 (phospho Tyr897) antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer
Dilution : 1:100
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GTX00795 WB Image
WB analysis of NIH-3T3 cell lysates using GTX00795 TIE2 (phospho Tyr897) antibody pre-incubated with phospho- or non-phospho- peptide.
P-peptide : phospho-peptide
N-peptide : non-phospho-peptide
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REFERENCE
There are currently no references for TIE2 (phospho Tyr897) antibody (GTX00795). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for TIE2 (phospho Tyr897) antibody (GTX00795). Be the first to share your experience with this product.
