GeneTex
United States (US)

TIE2 (phospho Tyr992) antibody

GTX55336_20151109_WB_18121411_162.jpg

Cat No. GTX55336

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human
APPLICATION

Application Note

WB: 1:500 - 1:1000

Calculated MW

126 kDa. ( Note )

Specificity/Sensitivity

The antibody detects endogenous levels of TIE2 only when phosphorylated at Tyr992.
PROPERTIES

Form

Liquid

Buffer

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of tyrosine 992 (E-V-Y(p)-V-K) derived from Human TIE2.

Purification

Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

TIE-2 Antibody , VMCM1 Antibody , TEK tyrosine kinase, endothelial Antibody , TEK Antibody , TIE2 Antibody , VMCM Antibody , CD202B Antibody

Background

The TEK receptor tyrosine kinase is expressed almost exclusively in endothelial cells in mice, rats, and humans. This receptor possesses a unique extracellular domain containing 2 immunoglobulin-like loops separated by 3 epidermal growth factor-like repeats that are connected to 3 fibronectin type III-like repeats. The ligand for the receptor is angiopoietin-1. Defects in TEK are associated with inherited venous malformations; the TEK signaling pathway appears to be critical for endothelial cell-smooth muscle cell communication in venous morphogenesis. TEK is closely related to the TIE receptor tyrosine kinase. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
GTX55336_20151109_WB_18121411_162.jpg

GTX55336 WB Image

Western blot analysis of extracts from HepG2 using TIE2 (Phospho-Tyr992) Antibody. The lane on the right is treated with the antigen-specific peptide.