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TIF1 alpha antibody [4G6C2]

Anti-TIF1 alpha antibody [4G6C2] used in IHC (Paraffin sections) (IHC-P). GTX04912
Anti-TIF1 alpha antibody [4G6C2] used in Western Blot (WB). GTX04912
Anti-TIF1 alpha antibody [4G6C2] used in IHC (Paraffin sections) (IHC-P). GTX04912
Anti-TIF1 alpha antibody [4G6C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04912
Anti-TIF1 alpha antibody [4G6C2] used in IHC (Paraffin sections) (IHC-P). GTX04912
Anti-TIF1 alpha antibody [4G6C2] used in IHC (Paraffin sections) (IHC-P). GTX04912

Cat. No. GTX04912

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 4G6C2

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μg ($469)
See all TIF1 alpha products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.25-0.5 μg/ml
ICC/IF 5 μg/ml
IHC-P 2-5 μg/ml
Not tested in other applications.

Calculated MW

117 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄

Preservative

No preservatives

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

E.coli-derived human TRIM24 recombinant protein (Position: A706-D964).

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

PTC6,RNF82,TF1A,TIF1,TIF1A,TIF1ALPHA,TRIM24,hTIF1,tripartite motif containing 24

Cellular Localization

Nucleus,Cytoplasm

Background

The protein encoded by this gene mediates transcriptional control by interaction with the activation function 2 (AF2) region of several nuclear receptors, including the estrogen, retinoic acid, and vitamin D3 receptors. The protein localizes to nuclear bodies and is thought to associate with chromatin and heterochromatin-associated factors. The protein is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains - a RING, a B-box type 1 and a B-box type 2 - and a coiled-coil region. Two alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-TIF1 alpha antibody [4G6C2] used in IHC (Paraffin sections) (IHC-P). GTX04912

GTX04912 IHC-P Image

IHC-P analysis of human breast cancer tissue using GTX04912 TIF1 alpha antibody [4G6C2].
Antigen retrieval : Heat mediated EDTA buffer, pH 8.0
Dilution : 2 μg/ml

Anti-TIF1 alpha antibody [4G6C2] used in Western Blot (WB). GTX04912

GTX04912 WB Image

WB analysis of various samples using GTX04912 TIF1 alpha antibody [4G6C2].
Loading : 30μg
Dilution : 0.5 μg/ml

Anti-TIF1 alpha antibody [4G6C2] used in IHC (Paraffin sections) (IHC-P). GTX04912

GTX04912 IHC-P Image

IHC-P analysis of human colon adenocarcinoma tissue using GTX04912 TIF1 alpha antibody [4G6C2].
Antigen retrieval : Heat mediated EDTA buffer, pH 8.0
Dilution : 2 μg/ml

Anti-TIF1 alpha antibody [4G6C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04912

GTX04912 ICC/IF Image

ICC/IF analysis of PFA-fixed MCF-7 cells using GTX04912 TIF1 alpha antibody [4G6C2].
Green : Primary antibody
Blue : DAPI
Antigen retrieval : IHC enzyme antigen retrieval reagent
Dilution : 5 μg/ml

Anti-TIF1 alpha antibody [4G6C2] used in IHC (Paraffin sections) (IHC-P). GTX04912

GTX04912 IHC-P Image

IHC-P analysis of human thyroid cancer tissue using GTX04912 TIF1 alpha antibody [4G6C2].
Antigen retrieval : Heat mediated EDTA buffer, pH 8.0
Dilution : 2 μg/ml

Anti-TIF1 alpha antibody [4G6C2] used in IHC (Paraffin sections) (IHC-P). GTX04912

GTX04912 IHC-P Image

IHC-P analysis of human ovarian serous tumor tissue using GTX04912 TIF1 alpha antibody [4G6C2].
Antigen retrieval : Heat mediated EDTA buffer, pH 8.0
Dilution : 2 μg/ml

REFERENCE

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REVIEW

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Package List Price ($)
$ 469