APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 4-6μg/ml |
|
Note :
IHC-P
Human Breast cancer shows nuclear staining in the distorted lobules.
|
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX89729-PEP).
Product Note
This antibody is expected to recognise both reported isoforms, as represented by NP_003843.3 and NP_056989.2.
Predict Reactivity
Bovine(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-KRLKSIEERQLLK, from the C Terminus of the protein sequence according to NP_056989.2; NP_003843.3.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
tripartite motif containing 24 , PTC6 , RNF82 , TF1A , TIF1 , TIF1A , TIF1ALPHA , hTIF1
Cellular Localization
Nucleus , Cytoplasm
Background
The protein encoded by this gene mediates transcriptional control by interaction with the activation function 2 (AF2) region of several nuclear receptors, including the estrogen, retinoic acid, and vitamin D3 receptors. The protein localizes to nuclear bodies and is thought to associate with chromatin and heterochromatin-associated factors. The protein is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains - a RING, a B-box type 1 and a B-box type 2 - and a coiled-coil region. Two alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
|
GTX89729 IHC-P Image
IHC-P analysis of human breast cancer using GTX89729 TIF1 alpha antibody, C-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 4μg/ml
|
REFERENCE
There are currently no references for TIF1 alpha antibody, C-term (GTX89729). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for TIF1 alpha antibody, C-term (GTX89729). Be the first to share your experience with this product.
