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TIF1 alpha antibody

Anti-TIF1 alpha antibody used in Flow cytometry (FACS). GTX04914
Anti-TIF1 alpha antibody used in Western Blot (WB). GTX04914

Cat. No. GTX04914

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, FACS, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μg ($469)
See all TIF1 alpha products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.1-0.25 μg/ml
FACS 1-3 μg/1x10⁶ cells
ELISA 0.1-0.5 μg/ml
Not tested in other applications.

Calculated MW

117 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄

Preservative

0.01 mg Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

E.coli-derived human TRIM24 recombinant protein (Position: A706-D964).

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

PTC6,RNF82,TF1A,TIF1,TIF1A,TIF1ALPHA,TRIM24,hTIF1,tripartite motif containing 24

Cellular Localization

Nucleus,Cytoplasm

Background

The protein encoded by this gene mediates transcriptional control by interaction with the activation function 2 (AF2) region of several nuclear receptors, including the estrogen, retinoic acid, and vitamin D3 receptors. The protein localizes to nuclear bodies and is thought to associate with chromatin and heterochromatin-associated factors. The protein is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains - a RING, a B-box type 1 and a B-box type 2 - and a coiled-coil region. Two alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-TIF1 alpha antibody used in Flow cytometry (FACS). GTX04914

GTX04914 FACS Image

FACS analysis of 4% PFA-fixed A431 cells using GTX04914 TIF1 alpha antibody.
Blue line : Primary antibody
Green line : Isotype Control
Red line : Blank control
Dilution : 1 μg/1x10⁶ cells

Anti-TIF1 alpha antibody used in Western Blot (WB). GTX04914

GTX04914 WB Image

WB analysis of various samples using GTX04914 TIF1 alpha antibody.
Loading : 50μg
Dilution : 0.25 μg/ml

REFERENCE

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Package List Price ($)
$ 469