TIF1 gamma antibody [6B7]

Cat. No. GTX83493

Host	Mouse
Clonality	Monoclonal
Clone Name	6B7
Isotype	IgG2b
Application	WB, ICC/IF, IHC-P
Reactivity	Human
Package	100 μl ($519)

See all TIF1 gamma products

Datasheet
Document

View product citations for antibody GTX83493 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500
ICC/IF	1:100
IHC-P	1:50

Not tested in other applications.

Calculated MW

123 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.85 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human recombinant protein fragment corresponding to amino acids 887-1046 of human Trim33 (NP_056990) produced in E.coli.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

tripartite motif containing 33 , ECTO , PTC7 , RFG7 , TF1G , TIF1G , TIF1GAMMA , TIFGAMMA

Cellular Localization

Nucleus

Background

Acts as an E3 ubiquitin-protein ligase. Promotes SMAD4 ubiquitination, nuclear exclusion and degradation via the ubiquitin proteasome pathway. According to PubMed:16751102, does not promote a decrease in the level of endogenous SMAD4. May act as a transcriptional repressor. Inhibits the transcriptional response to TGF-beta/BMP signaling cascade. Plays a role in the control of cell proliferation. Its association with SMAD2 and SMAD3 stimulates erythroid differentiation of hematopoietic stem/progenitor (By similarity). Monoubiquitinates SMAD4 and acts as an inhibitor of SMAD4-dependent TGF-beta/BMP signaling cascade (Monoubiquitination of SMAD4 hampers its ability to form a stable complex with activated SMAD2/3 resulting in inhibition of TGF-beta/BMP signaling cascade).

Database

Gene ID: 51592 TRIM33
UniProt: Q9UPN9 TRIM33

Research Area

DATA IMAGES

GTX83493 IHC-P Image

IHC-P analysis of human breast adenocarcinoma tissue using GTX83493 TIF1 gamma antibody [6B7].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

GTX83493 IHC-P Image

IHC-P analysis of human liver tissue using GTX83493 TIF1 gamma antibody [6B7].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

GTX83493 IHC-P Image

IHC-P analysis of human colon adenocarcinoma tissue using GTX83493 TIF1 gamma antibody [6B7].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

GTX83493 WB Image

WB analysis of HEK293T cells transfected with TIF1 gamma plasmid (Right) or empty vector (Left) for 48 hrs using GTX83493 TIF1 gamma antibody [6B7].
Loading : 5 ug per lane

GTX83493 IHC-P Image

IHC-P analysis of human kidney tissue using GTX83493 TIF1 gamma antibody [6B7].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

GTX83493 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with TIF1 gamma plasmid using GTX83493 TIF1 gamma antibody [6B7].

GTX83493 IHC-P Image

IHC-P analysis of human lung carcinoma tissue using GTX83493 TIF1 gamma antibody [6B7].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

GTX83493 IHC-P Image

IHC-P analysis of human ovary adenocarcinoma tissue using GTX83493 TIF1 gamma antibody [6B7].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μl

$ 519

Qty

TIF1 gamma antibody [6B7]

Cat. No. GTX83493

Host

Clonality

Clone Name

Isotype

Application

Reactivity

APPLICATION

Application Note

Calculated MW

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

Note

TARGET

Synonyms

Cellular Localization

Background

Database

Research Area

DATA IMAGES

GTX83493 IHC-P Image

GTX83493 IHC-P Image

GTX83493 IHC-P Image

GTX83493 WB Image

GTX83493 IHC-P Image

GTX83493 ICC/IF Image

GTX83493 IHC-P Image

GTX83493 IHC-P Image

REFERENCE

REVIEW

Product

Research

Support

Company

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