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TIF1 gamma antibody [9D4]

Anti-TIF1 gamma antibody [9D4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83492
Anti-TIF1 gamma antibody [9D4] used in Western Blot (WB). GTX83492
Anti-TIF1 gamma antibody [9D4] used in IHC (Paraffin sections) (IHC-P). GTX83492
Anti-TIF1 gamma antibody [9D4] used in IHC (Paraffin sections) (IHC-P). GTX83492
Anti-TIF1 gamma antibody [9D4] used in IHC (Paraffin sections) (IHC-P). GTX83492
Anti-TIF1 gamma antibody [9D4] used in IHC (Paraffin sections) (IHC-P). GTX83492
Anti-TIF1 gamma antibody [9D4] used in IHC (Paraffin sections) (IHC-P). GTX83492
Anti-TIF1 gamma antibody [9D4] used in IHC (Paraffin sections) (IHC-P). GTX83492
Anti-TIF1 gamma antibody [9D4] used in IHC (Paraffin sections) (IHC-P). GTX83492
Anti-TIF1 gamma antibody [9D4] used in IHC (Paraffin sections) (IHC-P). GTX83492
Anti-TIF1 gamma antibody [9D4] used in IHC (Paraffin sections) (IHC-P). GTX83492
Anti-TIF1 gamma antibody [9D4] used in IHC (Paraffin sections) (IHC-P). GTX83492
Anti-TIF1 gamma antibody [9D4] used in IHC (Paraffin sections) (IHC-P). GTX83492

Cat. No. GTX83492

Host

Mouse

Clonality

Monoclonal

Clone Name

9D4

Isotype

IgG2a

Application

WB, ICC/IF, IHC-P

Reactivity

Human
Package
100 μl ($519)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500
ICC/IF 1:100
IHC-P 1:50
Not tested in other applications.

Calculated MW

123 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human recombinant protein fragment corresponding to amino acids 887-1046 of human Trim33 (NP_056990) produced in E.coli.

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

tripartite motif containing 33 , ECTO , PTC7 , RFG7 , TF1G , TIF1G , TIF1GAMMA , TIFGAMMA

Cellular Localization

Nucleus

Background

Acts as an E3 ubiquitin-protein ligase. Promotes SMAD4 ubiquitination, nuclear exclusion and degradation via the ubiquitin proteasome pathway. According to PubMed:16751102, does not promote a decrease in the level of endogenous SMAD4. May act as a transcriptional repressor. Inhibits the transcriptional response to TGF-beta/BMP signaling cascade. Plays a role in the control of cell proliferation. Its association with SMAD2 and SMAD3 stimulates erythroid differentiation of hematopoietic stem/progenitor (By similarity). Monoubiquitinates SMAD4 and acts as an inhibitor of SMAD4-dependent TGF-beta/BMP signaling cascade (Monoubiquitination of SMAD4 hampers its ability to form a stable complex with activated SMAD2/3 resulting in inhibition of TGF-beta/BMP signaling cascade).

Database

Research Area

DATA IMAGES

Anti-TIF1 gamma antibody [9D4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83492

GTX83492 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with TIF1 gamma plasmid using GTX83492 TIF1 gamma antibody [9D4].

Anti-TIF1 gamma antibody [9D4] used in Western Blot (WB). GTX83492

GTX83492 WB Image

WB analysis of HEK293T cells transfected with TIF1 gamma plasmid (Right) or empty vector (Left) for 48 hrs using GTX83492 TIF1 gamma antibody [9D4].
Loading : 5 ug per lane

Anti-TIF1 gamma antibody [9D4] used in IHC (Paraffin sections) (IHC-P). GTX83492

GTX83492 IHC-P Image

IHC-P analysis of human kidney tissue using GTX83492 TIF1 gamma antibody [9D4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-TIF1 gamma antibody [9D4] used in IHC (Paraffin sections) (IHC-P). GTX83492

GTX83492 IHC-P Image

IHC-P analysis of human ovary tissue using GTX83492 TIF1 gamma antibody [9D4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-TIF1 gamma antibody [9D4] used in IHC (Paraffin sections) (IHC-P). GTX83492

GTX83492 IHC-P Image

IHC-P analysis of human thyroid carcinoma tissue using GTX83492 TIF1 gamma antibody [9D4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-TIF1 gamma antibody [9D4] used in IHC (Paraffin sections) (IHC-P). GTX83492

GTX83492 IHC-P Image

IHC-P analysis of human colon adenocarcinoma tissue using GTX83492 TIF1 gamma antibody [9D4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-TIF1 gamma antibody [9D4] used in IHC (Paraffin sections) (IHC-P). GTX83492

GTX83492 IHC-P Image

IHC-P analysis of human breast tissue using GTX83492 TIF1 gamma antibody [9D4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-TIF1 gamma antibody [9D4] used in IHC (Paraffin sections) (IHC-P). GTX83492

GTX83492 IHC-P Image

IHC-P analysis of human kidney carcinoma tissue using GTX83492 TIF1 gamma antibody [9D4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-TIF1 gamma antibody [9D4] used in IHC (Paraffin sections) (IHC-P). GTX83492

GTX83492 IHC-P Image

IHC-P analysis of human colon tissue using GTX83492 TIF1 gamma antibody [9D4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-TIF1 gamma antibody [9D4] used in IHC (Paraffin sections) (IHC-P). GTX83492

GTX83492 IHC-P Image

IHC-P analysis of human breast adenocarcinoma tissue using GTX83492 TIF1 gamma antibody [9D4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-TIF1 gamma antibody [9D4] used in IHC (Paraffin sections) (IHC-P). GTX83492

GTX83492 IHC-P Image

IHC-P analysis of human lung carcinoma tissue using GTX83492 TIF1 gamma antibody [9D4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-TIF1 gamma antibody [9D4] used in IHC (Paraffin sections) (IHC-P). GTX83492

GTX83492 IHC-P Image

IHC-P analysis of human ovary adenocarcinoma tissue using GTX83492 TIF1 gamma antibody [9D4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-TIF1 gamma antibody [9D4] used in IHC (Paraffin sections) (IHC-P). GTX83492

GTX83492 IHC-P Image

IHC-P analysis of human pancreas carcinoma tissue using GTX83492 TIF1 gamma antibody [9D4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519