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TIF1 gamma antibody

Anti-TIF1 gamma antibody used in Western Blot (WB). GTX131686
Anti-TIF1 gamma antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX131686

Cat No. GTX131686

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

123 kDa. ( Note )

Positive Control

MCF-7 , MDA-MB-231

Predict Reactivity

Mouse, Bovine, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2.21 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of Human TIF1 gamma. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

tripartite motif containing 33,ECTO,PTC7,RFG7,TF1G,TIF1G,TIF1GAMMA,TIFGAMMA

Cellular Localization

Nucleus

Background

The protein encoded by this gene is thought to be a transcriptional corepressor. However, molecules that interact with this protein have not yet been identified. The protein is a member of the tripartite motif family. This motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. Three alternatively spliced transcript variants for this gene have been described, however, the full-length nature of one variant has not been determined. [provided by RefSeq, Jul 2008]

Database

DATA IMAGES
Anti-TIF1 gamma antibody used in Western Blot (WB). GTX131686

GTX131686 WB Image

Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with TIF1 gamma antibody (GTX131686) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TIF1 gamma antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX131686

GTX131686 ICC/IF Image

TIF1 gamma antibody detects TIF1 gamma protein at nucleus by immunofluorescent analysis.
Sample: MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TIF1 gamma stained by TIF1 gamma antibody (GTX131686) diluted at 1:2000.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500.

REFERENCE
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REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169