APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
MCF-7 , MDA-MB-231
Predict Reactivity
Mouse, Bovine, Chicken, Xenopus tropicalis(>80% identity)
PROPERTIES
Form
Liquid
Buffer
1XPBS (pH7), 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
2.21 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human TIF1 gamma. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2886514
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
tripartite motif containing 33 , ECTO , PTC7 , RFG7 , TF1G , TIF1G , TIF1GAMMA , TIFGAMMA
Cellular Localization
Nucleus
Background
The protein encoded by this gene is thought to be a transcriptional corepressor. However, molecules that interact with this protein have not yet been identified. The protein is a member of the tripartite motif family. This motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. Three alternatively spliced transcript variants for this gene have been described, however, the full-length nature of one variant has not been determined. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX131686 ICC/IF Image
TIF1 gamma antibody detects TIF1 gamma protein at nucleus by immunofluorescent analysis.Sample: MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: TIF1 gamma stained by TIF1 gamma antibody (GTX131686) diluted at 1:2000.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500.
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GTX131686 IHC-P Image
TIF1 gamma antibody detects TIF1 gamma protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded human breast carcinoma.TIF1 gamma stained by TIF1 gamma antibody (GTX131686) diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX131686 WB Image
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with TIF1 gamma antibody (GTX131686) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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REFERENCE
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REVIEW
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