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TIGAR antibody [C2C3], C-term

Anti-TIGAR antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX110514
Anti-TIGAR antibody [C2C3], C-term used in Western Blot (WB). GTX110514

Cat. No. GTX110514

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all TIGAR products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

30 kDa. ( Note )

Positive Control

HepG2 , Molt-4

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human TIGAR. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949774

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

TP53 induced glycolysis regulatory phosphatase , C12orf5 , FR2BP

Background

This gene is regulated as part of the p53 tumor suppressor pathway and encodes a protein with sequence similarity to the bisphosphate domain of the glycolytic enzyme that degrades fructose-2,6-bisphosphate. The protein functions by blocking glycolysis and directing the pathway into the pentose phosphate shunt. Expression of this protein also protects cells from DNA damaging reactive oxygen species and provides some protection from DNA damage-induced apoptosis. The 12p13.32 region that includes this gene is paralogous to the 11q13.3 region. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-TIGAR antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX110514

GTX110514 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Hela xenograft, using TIGAR(GTX110514) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-TIGAR antibody [C2C3], C-term used in Western Blot (WB). GTX110514

GTX110514 WB Image

Sample(30 μg whole cell lysate)
A:Hep G2 (GTX27900)
B:MOLT4 (GTX27912)
12% SDS PAGE
GTX110514 diluted at 1:3000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Consoli V et al. Int J Mol Sci 2023; 24 (13) Evaluation of the Antioxidant and Antiangiogenic Activity of a Pomegranate Extract in BPH-1 Prostate Epithelial Cells.
Sorrenti V et al. Biomolecules 2021; 11 (6) Tin Mesoporphyrin Selectively Reduces Non-Small-Cell Lung Cancer Cell Line A549 Proliferation by Interfering with Heme Oxygenase and Glutathione Systems.
Application : WB
Reactivity : Human
Fang P et al. Commun Biol 2019; 2 : 335 Genome-scale CRISPR knockout screen identifies TIGAR as a modifier of PARP inhibitor sensitivity.
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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169