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TIGAR antibody

Anti-TIGAR antibody used in IHC (Paraffin sections) (IHC-P). GTX31307
Anti-TIGAR antibody used in IHC (Paraffin sections) (IHC-P). GTX31307
Anti-TIGAR antibody used in Western Blot (WB). GTX31307

Cat No. GTX31307

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P, ELISA

Reactivity

Human, Mouse
Package
100 μg ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.5 - 2 μg/mL
IHC-P 2.5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

30 kDa. ( Note )

Positive Control

Human Brain Tissue Slide(GTX22203) , Human Brain Tissue Lysate(GTX27918) , MCF7 Cell Lysate

PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

TIGAR antibody was raised against a 19 amino acid synthetic peptide from near the center of human TIGAR.The immunogen is located within amino acids 100 - 150 of TIGAR.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

TP53 induced glycolysis regulatory phosphatase , C12orf5 , FR2BP

Cellular Localization

Cytoplasm,Nucleus,Mitochondrion

Background

This gene is regulated as part of the p53 tumor suppressor pathway and encodes a protein with sequence similarity to the bisphosphate domain of the glycolytic enzyme that degrades fructose-2,6-bisphosphate. The protein functions by blocking glycolysis and directing the pathway into the pentose phosphate shunt. Expression of this protein also protects cells from DNA damaging reactive oxygen species and provides some protection from DNA damage-induced apoptosis. The 12p13.32 region that includes this gene is paralogous to the 11q13.3 region. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-TIGAR antibody used in IHC (Paraffin sections) (IHC-P). GTX31307

GTX31307 IHC-P Image

IHC-P analysis of human brain tissue using GTX31307 TIGAR antibody.
Working concentration : 20 μg/ml

Anti-TIGAR antibody used in IHC (Paraffin sections) (IHC-P). GTX31307

GTX31307 IHC-P Image

IHC-P analysis of human brain tissue using GTX31307 TIGAR antibody.
Working concentration : 2.5 μg/ml

Anti-TIGAR antibody used in Western Blot (WB). GTX31307

GTX31307 WB Image

WB analysis of MCF7 cell lysate using GTX31307 TIGAR antibody.
Working concentration : (A) 0.5, (B) 1, and (C) 2 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 339