Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membranes were blotted with TIGIT antibody [HL2655] (GTX639114) diluted at 1:1000 and competitor's antibody (Highly Cited Antibody) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with TIGIT antibody [HL2655] (GTX639114) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

MJ whole cell and membrane extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with TIGIT antibody [HL2655] (GTX639114) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with TIGIT antibody [HL2655] (GTX639114) diluted at 1:30000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.