APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
0.3-1μg/ml |
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX89379-PEP).
Predict Reactivity
Dog(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-KWYSHSKEKIQN, from the internal region of the protein sequence according to NP_116171.3.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
hepatitis A virus cellular receptor 2 , CD366 , HAVcr-2 , KIM-3 , TIM3 , TIMD-3 , TIMD3 , Tim-3
Cellular Localization
Membrane , Cell junction
Background
The protein encoded by this gene belongs to the immunoglobulin superfamily, and TIM family of proteins. CD4-positive T helper lymphocytes can be divided into types 1 (Th1) and 2 (Th2) on the basis of their cytokine secretion patterns. Th1 cells are involved in cell-mediated immunity to intracellular pathogens and delayed-type hypersensitivity reactions, whereas, Th2 cells are involved in the control of extracellular helminthic infections and the promotion of atopic and allergic diseases. This protein is a Th1-specific cell surface protein that regulates macrophage activation, and inhibits Th1-mediated auto- and alloimmune responses, and promotes immunological tolerance. [provided by RefSeq, Sep 2011]
Database
Research Area
DATA IMAGES
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GTX89379 WB Image
WB analysis of HEK293 overexpressing TIM-3 (mock transfection in first lane) using GTX89379 TIM-3 antibody, Internal.
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GTX89379 WB Image
WB analysis of human tonsil lysate using GTX89379 TIM-3 antibody, Internal. Dilution : 0.3μg/ml Loading : 35μg protein in RIPA buffer
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REFERENCE
There are currently no references for TIM-3 antibody, Internal (GTX89379). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for TIM-3 antibody, Internal (GTX89379). Be the first to share your experience with this product.