GeneTex
United States (US)

TIM17 antibody [C1C3]

GTX108280_WB_19011507_828.jpg
GTX108280_39750_IFA_19011507_546.jpg

Cat No. GTX108280

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

18 kDa. ( Note )

Positive Control

A431 , H1299 , HeLa

Predict Reactivity

Rat, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human TIM17. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Translocase Of Inner Mitochondrial Membrane 17A,Tim17,Tim17A,Timm17A

Cellular Localization

Mitochondrion inner membrane; Multi-pass membrane protein

Background

Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane.

Database

Research Area

DATA IMAGES
GTX108280_WB_19011507_828.jpg

GTX108280 WB Image

Sample (30 µg of whole cell lysate)
A: HeLa
12% SDS PAGE
GTX108280 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX108280_39750_IFA_19011507_546.jpg

GTX108280 ICC/IF Image

TIM17 antibody detects TIM17 protein at mitochondria by immunofluorescent analysis.
Sample: A549 cells were fixed in ice-cold MeOH for 5 min.
Green: TIM17 protein stained by TIM17 antibody (GTX108280) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169