GeneTex

  • Country / Location Selection

United States (US)

TIMP2 antibody [3A4]

Anti-TIMP2 antibody [3A4] used in IHC (Paraffin sections) (IHC-P). GTX35122

Cat. No. GTX35122

Reference 4

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 3A4

Isotype

 IgG2a

Application

 WB, ICC/IF, IHC-P, FACS

Reactivity

 Human, Mouse, Rat, Zebrafish, Rabbit, Bovine, Guinea pig
Package
100 μg ($529)
See all TIMP2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1-2μg/ml
ICC/IF 1-2μg/ml
IHC-P 1-2μg/ml for 30 minutes at RT
FACS 1-2μg/10⁶ cells

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
Not tested in other applications.

Calculated MW

24 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide from the N-terminus of human TIMP2 protein (aa56-66) (DSGNDIYGNPI)

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CSC21K , DDC8 , TIMP metallopeptidase inhibitor 2 , TIMP2

Cellular Localization

Secreted

Background

This gene is a member of the TIMP gene family. The proteins encoded by this gene family are natural inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix. In addition to an inhibitory role against metalloproteinases, the encoded protein has a unique role among TIMP family members in its ability to directly suppress the proliferation of endothelial cells. As a result, the encoded protein may be critical to the maintenance of tissue homeostasis by suppressing the proliferation of quiescent tissues in response to angiogenic factors, and by inhibiting protease activity in tissues undergoing remodelling of the extracellular matrix. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-TIMP2 antibody [3A4] used in IHC (Paraffin sections) (IHC-P). GTX35122

GTX35122 IHC-P Image

IHC-P analysis of human placenta tissue using GTX35122 TIMP2 antibody [3A4].

REFERENCE

Clone Reference

Wang J et al. Neoplasia 2019; 21 (9):908-920 p85α Inactivates MMP-2 and Suppresses Bladder Cancer Invasion by Inhibiting MMP-14 Transcription and TIMP-2 Degradation.
Bu S et al. Arch Biochem Biophys 2014; Simulated physiological stretch-induced proliferation of human bladder smooth muscle cells is regulated by MMPs.
Lin YC et al. PLoS One 2013; 8 (8):e71903 Impact of flavonoids on matrix metalloproteinase secretion and invadopodia formation in highly invasive A431-III cancer cells.
Corradi D et al. J Cardiovasc Electrophysiol 2012; 23 (3):271-9 Differential structural remodeling of the left-atrial posterior wall in patients affected by mitral regurgitation with or without persistent atrial fibrillation: a morphological and molecular study.
Submit a Reference

REVIEW

There are currently no reviews for TIMP2 antibody [3A4] (GTX35122). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 529